CAJ | 학술논문

目的：通过对ApoE基因敲除小鼠喂食搜风祛痰中药复方稳斑汤后观察动脉粥样硬化（AS）不稳定斑块自噬相关蛋白Beclin-1、Bcl-2、 LC3的改变，探索治疗动脉粥样硬化新思路、新方法。方法将ApoE基因敲除小鼠动脉粥样硬化不稳定斑块模型随机分成空白组、模型组、稳斑汤低、中、高剂量组、西药组。小鼠麻醉处死取主动脉组织HE染色后进行病理学观察，采用半定量 RT-PCR 技术检测Beclin-1、Bcl-2、LC3蛋白表达变化。结果与空白组比较，模型组小鼠腹主动脉组织中Beclin-1、Bcl-2、LC3蛋白表达水平显著降低（P<0.01）；与模型组比较，西药组和稳斑汤各剂量组均能提高Beclin-1、Bcl-2、LC3表达水平（P<0.01）；稳斑汤高剂量组与西药组在Beclin-1、Bcl-2、LC3表达水平上差异无统计学意义（P>0.05）。结论中药搜风祛痰法稳斑汤可以显著提高Beclin-1、Bcl-2、LC3蛋白表达水平，从而达到稳定动脉硬化斑块、抗动脉硬化效果。
목적：통과대ApoE기인고제소서위식수풍거담중약복방은반탕후관찰동맥죽양경화（AS）불은정반괴자서상관단백Beclin-1、Bcl-2、 LC3적개변，탐색치료동맥죽양경화신사로、신방법。방법장ApoE기인고제소서동맥죽양경화불은정반괴모형수궤분성공백조、모형조、은반탕저、중、고제량조、서약조。소서마취처사취주동맥조직HE염색후진행병이학관찰，채용반정량 RT-PCR 기술검측Beclin-1、Bcl-2、LC3단백표체변화。결과여공백조비교，모형조소서복주동맥조직중Beclin-1、Bcl-2、LC3단백표체수평현저강저（P<0.01）；여모형조비교，서약조화은반탕각제량조균능제고Beclin-1、Bcl-2、LC3표체수평（P<0.01）；은반탕고제량조여서약조재Beclin-1、Bcl-2、LC3표체수평상차이무통계학의의（P>0.05）。결론중약수풍거담법은반탕가이현저제고Beclin-1、Bcl-2、LC3단백표체수평，종이체도은정동맥경화반괴、항동맥경화효과。
ObjectiveTo observe the effects of Soufengqutan Chinese herbal compound Wenban Decoction on ApoE knockout mice AS unstable plaque animal models.Methods Tobuild the ApoE knockout mice AS unstable plaque animal models and divide them into blank control group,model control group,Western medicine group and Wenban Decoction low,middle and high dose group.After the implementation of anesthesia and execution,take out the aorta tissue in mice which should be HE stained and then pathologically observed under the optical microscope.Then detect the expression changes of Beclin-1 Bcl-2 and LC3 with semi- quantitative RT-PCR technique.Results Beclin-1 LC3 expression levels in the model group were significantly lower than those of normal group(P<0.01).Bcl-2 expression levels in the model group were significantly lower than those of normal group(P<0.05).Compared with model group,Beclin-1 Bcl-2 and LC3 expression levels in each medicated group were significantly higher (P<0.01).There was no difference between Western medicine group and Wenban Decoction high dose group in expression levels of Beclin-1 Bcl-2 and LC3 (P>0.05).Conclution Soufengqutan Chinese herbal compound Wenban Decoction may intervene the formation and eruption of AS unstable plaque through up-regulating the expression of Beclin-1 Bcl-2 and LC3 in ApoE knockout mice AS unstable plaque,which makes the cardiovascular system well protected.