CAJ | 학술논문

目的：检测急性肾小管肾炎患者血清抗钠泵α1亚基抗体的水平，并评价其对钠泵功能的影响。方法急性肾小球肾炎患儿195例，并对其中33例建立随访，分别于疾病期间及疾病痊愈后3个月和6个月检测血清抗钠泵α1亚基抗体的水平。同时使用受试者血清作用 HEK -293A 细胞膜蛋白后检测钠泵活性。结果急性肾小球肾炎患者血清抗钠泵α1亚基抗体水平较对照组显著升高（ P ﹤0.05），女性显著高于男性（ P ﹤0.05），且主要表现为 IgM 类抗体升高。3个月后血清 IgM 抗钠泵α1亚基抗体水平较患病时显著下降（ P ﹤0.05），但 IgG 抗钠泵α1亚基抗体水平较患病时显著升高（ P ﹤0.05）。6个月后 IgM 抗体平均降至表面健康对照组水平，但 IgG 类抗体水平仍高于对照组（ P ﹤0.05）。细胞实验结果表明，IgG 类抗钠泵α1亚基能显著抑制人肾脏细胞系 HEK -293A 细胞的钠泵活性（ P ﹤0.05），但 IgM 类抗体无上述作用（ P ﹥0.05）。结论急性肾小球肾炎患者血清中可存在钠泵α1亚基自身抗体。IgG 类钠泵α1亚基自身抗体能抑制钠泵的活性，这可能是导致急性肾小球肾炎慢性长期肾损伤的机制之一。
목적：검측급성신소관신염환자혈청항납빙α1아기항체적수평，병평개기대납빙공능적영향。방법급성신소구신염환인195례，병대기중33례건립수방，분별우질병기간급질병전유후3개월화6개월검측혈청항납빙α1아기항체적수평。동시사용수시자혈청작용 HEK -293A 세포막단백후검측납빙활성。결과급성신소구신염환자혈청항납빙α1아기항체수평교대조조현저승고（ P ﹤0.05），녀성현저고우남성（ P ﹤0.05），차주요표현위 IgM 류항체승고。3개월후혈청 IgM 항납빙α1아기항체수평교환병시현저하강（ P ﹤0.05），단 IgG 항납빙α1아기항체수평교환병시현저승고（ P ﹤0.05）。6개월후 IgM 항체평균강지표면건강대조조수평，단 IgG 류항체수평잉고우대조조（ P ﹤0.05）。세포실험결과표명，IgG 류항납빙α1아기능현저억제인신장세포계 HEK -293A 세포적납빙활성（ P ﹤0.05），단 IgM 류항체무상술작용（ P ﹥0.05）。결론급성신소구신염환자혈청중가존재납빙α1아기자신항체。IgG 류납빙α1아기자신항체능억제납빙적활성，저가능시도치급성신소구신염만성장기신손상적궤제지일。
Objective To detect the serum Na pump α1 submit autoantibody in pediatric patients with acute glomerulonephritis and to e-valuate its effect on activity of Na pump. Methods A total of 195 pediatric patients with acute glomerulonephritis were enrolled in this study. A-mong them,33 cases had established follow - up during thecourse of illness and three months to six months after recovery of the disease. Results The serum level of Na pump α1 subunit antibody in patients with acute glomerulonephritis was significantly higher than that of control group( P﹤ 0. 05),the percentage of affection in girls were significantly higher than that of boys( P ﹤ 0. 05),and it mainlypresented in IgM class antibod-ies. Serum Na pump α1 IgM antibody was significantly decreased in 3 months after onset of the disease( P ﹤ 0. 05),but serum level of IgG anti-body was significantly increased than that of controls( P ﹤ 0. 05). Serum level of Na pump α1 IgG antibody was significantly higher than that of controls after 6 months( P ﹤ 0. 05)and serum level of IgM antibody was similar to that of controls. IgG Na pump α1 subunit antibody could sig-nificantly inhibit the activity of Na pump in HEK - 293A cells( P ﹤ 0. 05),but IgM antibody had no such effect( P ﹥ 0. 05). Conclusion Na pump α1 subunit autoantibodies can be detected in pediatric patients with acute glomerulonephritis. IgG Na pump α1 subunit autoantibody can in-hibit the activity of Na pump,and it may play animportant role in long - term renal impairment in patients with acute glomerulonephritis.