实用医学杂志
實用醫學雜誌
실용의학잡지
The Journal of Practical Medicine
2015年
17期
2815-2818
,共4页
罗炜%杨超培%王慧%陈如冲
囉煒%楊超培%王慧%陳如遲
라위%양초배%왕혜%진여충
嗜酸粒细胞性支气管炎%MUC5AC%MUC5B%LTC4%PGE2
嗜痠粒細胞性支氣管炎%MUC5AC%MUC5B%LTC4%PGE2
기산립세포성지기관염%MUC5AC%MUC5B%LTC4%PGE2
Eosinophilic bronchitis%MUC5AC%MUC5B%LTC4%PGE2
目的：分析嗜酸粒细胞性支气管炎（EB）与哮喘患者的诱导痰黏蛋白水平的差异并探讨其差异机制。方法：通过ELISA法检测20例EB、哮喘患者及正常对照的痰上清液黏蛋白 MUC5AC、MUC5B及LTC4和PGE2水平，分析上述炎症介质在EB与哮喘患者的黏蛋白差异机制中所起的作用。结果：哮喘组的MUC5AC[（26．8±8．5）μg／mL]显著高于EB组[（17．6±7．0）μg／mL]和对照组[（12．5±4．3）μg／mL]（P ＜0．01），MUC5B[（1．1±0．5）μg／mL]与EB组[（2．6±0．5）μg／mL]和对照组[（2．6±0．4）μg／mL]相比，显著降低（P＜0．01），EB组的MUC5AC和MUC5B水平与对照组比较差异无显著性（P ＞0．05）。与其他组相比，哮喘组的LTC4[（700．8±172．3）pg／mL]和EB组的PGE2[（433．8±118．1）pg／mL]均显著升高（P ＜0．01）。受试者的痰上清LTC4与MUC5AC呈正相关（r＝0．785，P＜0．01），与MUC5B呈负相关（r＝－0．703，P＜0．01）。结论：哮喘与EB患者的大气道黏蛋白MUC5AC与MUC5B的比例存在显著差异，而LTC4和PGE2之间的失衡可能是引起该差异的机制。
目的：分析嗜痠粒細胞性支氣管炎（EB）與哮喘患者的誘導痰黏蛋白水平的差異併探討其差異機製。方法：通過ELISA法檢測20例EB、哮喘患者及正常對照的痰上清液黏蛋白 MUC5AC、MUC5B及LTC4和PGE2水平，分析上述炎癥介質在EB與哮喘患者的黏蛋白差異機製中所起的作用。結果：哮喘組的MUC5AC[（26．8±8．5）μg／mL]顯著高于EB組[（17．6±7．0）μg／mL]和對照組[（12．5±4．3）μg／mL]（P ＜0．01），MUC5B[（1．1±0．5）μg／mL]與EB組[（2．6±0．5）μg／mL]和對照組[（2．6±0．4）μg／mL]相比，顯著降低（P＜0．01），EB組的MUC5AC和MUC5B水平與對照組比較差異無顯著性（P ＞0．05）。與其他組相比，哮喘組的LTC4[（700．8±172．3）pg／mL]和EB組的PGE2[（433．8±118．1）pg／mL]均顯著升高（P ＜0．01）。受試者的痰上清LTC4與MUC5AC呈正相關（r＝0．785，P＜0．01），與MUC5B呈負相關（r＝－0．703，P＜0．01）。結論：哮喘與EB患者的大氣道黏蛋白MUC5AC與MUC5B的比例存在顯著差異，而LTC4和PGE2之間的失衡可能是引起該差異的機製。
목적：분석기산립세포성지기관염（EB）여효천환자적유도담점단백수평적차이병탐토기차이궤제。방법：통과ELISA법검측20례EB、효천환자급정상대조적담상청액점단백 MUC5AC、MUC5B급LTC4화PGE2수평，분석상술염증개질재EB여효천환자적점단백차이궤제중소기적작용。결과：효천조적MUC5AC[（26．8±8．5）μg／mL]현저고우EB조[（17．6±7．0）μg／mL]화대조조[（12．5±4．3）μg／mL]（P ＜0．01），MUC5B[（1．1±0．5）μg／mL]여EB조[（2．6±0．5）μg／mL]화대조조[（2．6±0．4）μg／mL]상비，현저강저（P＜0．01），EB조적MUC5AC화MUC5B수평여대조조비교차이무현저성（P ＞0．05）。여기타조상비，효천조적LTC4[（700．8±172．3）pg／mL]화EB조적PGE2[（433．8±118．1）pg／mL]균현저승고（P ＜0．01）。수시자적담상청LTC4여MUC5AC정정상관（r＝0．785，P＜0．01），여MUC5B정부상관（r＝－0．703，P＜0．01）。결론：효천여EB환자적대기도점단백MUC5AC여MUC5B적비례존재현저차이，이LTC4화PGE2지간적실형가능시인기해차이적궤제。
Objective To investigated the difference in induced sputum concentrations of mucin between the patients with eosinophilic bronchitis (EB) and classic asthma, and found out the difference in mechanism. Methods 20 patients with eosinophilic bronchitis (EB), 20 patients with classic asthma and 10 healthy controls were enrolled. Induced sputum levels of MUC5AC, MUC5B, LTC4 and PGE2 were measured by enzyme immunoassay in patients and healthy control. Results In patients with asthma, sputum MUC5A (26.8 ± 8.5)μg/mL and LTC4 (700.8 ± 172.3)pg/mL were significantly elevated whereas MUC5B levels (1.1 ± 0.5)μg/mL were reduced compared with patients with EB MUC5AC:( 17 . 6 ± 7 . 0 )μg/mL , LTC4:( 320 . 9 ± 89 . 4 ) pg/mL , MUC5B:(2.6 ± 0.5)μg/mL and healthy controls MUC5AC:(12.5 ± 4.3)μg/mL, LTC4:(91.2 ± 16.1)pg/mL, MUC5B:(2.6 ± 0.4)μg/mL (P < 0.01). PGE2 levels were higher in patients with EB (433.8 ± 118.1)pg/mL than in asthma(172.7 ± 74.5)pg/mL and controls(113.3 ± 18.3)pg/mL(P < 0.01), but results did not differ between asthma and controls. The LTC4 showed a significant positive correlation with MUC5AC (r = 0.785,P < 0.01), and a significant negative correlation with MUC5B (r = -0.703,P < 0.01). Conclusion A significant difference in MUC5AC/MUC5B ratio was founded in proximal airway between EB and asthma. The unbalance level between LTC4 and PGE2 would be involved in the pathogenesis of airway mucus hypersecretion.
