CAJ | 학술논문

以红景天为原料,采用微波辅助的方法优化了红景天苷和酪醇的提取工艺. 先采用单因素实验考察了提取剂种类∕浓度、温度、时间、液固比对目标物提取的影响,然后选择提取时间、乙醇浓度、液固比3个因素为自变量, 采用响应曲面法的Box-Behnken中心组合试验设计研究各因素及其交互作用对红景天苷和酪醇提取率的影响,同时建立响应曲面回归模型用以估计红景天苷和酪醇的最佳提取条件,得到最佳提取工艺条件为提取时间35 min、乙醇质量分数20%、液固比10:1;在此条件下,做3次平行实验,红景天苷和酪醇提取率为0.60%、0.16%,与模型预测值基本相符.
이홍경천위원료,채용미파보조적방법우화료홍경천감화락순적제취공예. 선채용단인소실험고찰료제취제충류∕농도、온도、시간、액고비대목표물제취적영향,연후선택제취시간、을순농도、액고비3개인소위자변량, 채용향응곡면법적Box-Behnken중심조합시험설계연구각인소급기교호작용대홍경천감화락순제취솔적영향,동시건립향응곡면회귀모형용이고계홍경천감화락순적최가제취조건,득도최가제취공예조건위제취시간35 min、을순질량분수20%、액고비10:1;재차조건하,주3차평행실험,홍경천감화락순제취솔위0.60%、0.16%,여모형예측치기본상부.
A microwave-assisted extraction method of salidroside and tyrosol from the Rhodiola was optimized by response surface method (RSM). The extraction process were simulated and the main factors affecting the extraction,such as the kinds and concentrations of solvent,liquid∕solid ratio of solvent to sample,irradiation time,irradiation temperature were investigated. Based on single factor experiments,Box-Behnken central com-posite experimental design of RSM was used to explore the effects of extraction time,ethanol concentration, liquid-solid ratio and their cross-interactions on the yields of salidroside and tyrosol. Two extraction regres-sion equations of salidroside and tyrosol were established respectively through experiments and the optimal conditions obtained by RSM are extraction time of 35 min,ethanol concentration of 20%,solid-liquid ratio of 1:10. The yields of salidroside and tyrosol are 0.597 2% and 0.158%,respectively,which is well agreement with the yield predicted by the model.