CAJ | 학술논문

目的：探索抑制着丝粒蛋白F（CENP－F）表达对人胶质母细胞瘤细胞系U251增殖的影响。方法：培养U251细胞，转染抑制片段，检测空白对照组（未做任何处理，Blank组），阴性对照组（转入空白质粒组，NC组）及转入抑制片段组（si－CENPF组，根据转入的干扰片段分si－CENPF＃1组、si－CENPF＃2组、si－CENPF＃3组）各组CENP－F mRNA的表达。挑选抑制最明显的片段进行Western－botting进一步检测CENP－F蛋白水平的表达。行MTT检测转染细胞组与空白对照组及阴性对照组的细胞增殖力。结果：干扰片段能抑制U251细胞系CENP－F mRNA水平及蛋白水平的表达，同时能抑制U251细胞的增殖。结论：抑制CEAFP的表达能降低人胶质母细胞瘤细胞系U251的增殖。
목적：탐색억제착사립단백F（CENP－F）표체대인효질모세포류세포계U251증식적영향。방법：배양U251세포，전염억제편단，검측공백대조조（미주임하처리，Blank조），음성대조조（전입공백질립조，NC조）급전입억제편단조（si－CENPF조，근거전입적간우편단분si－CENPF＃1조、si－CENPF＃2조、si－CENPF＃3조）각조CENP－F mRNA적표체。도선억제최명현적편단진행Western－botting진일보검측CENP－F단백수평적표체。행MTT검측전염세포조여공백대조조급음성대조조적세포증식력。결과：간우편단능억제U251세포계CENP－F mRNA수평급단백수평적표체，동시능억제U251세포적증식。결론：억제CEAFP적표체능강저인효질모세포류세포계U251적증식。
Objective:To investigate the effect of down-regulation of CENP-F by RNAi on proliferation in human glioma cell line U251. Methods: U251 cell line down-regulating CENP-F by RNAi was established. Forty-eight hours after transient transfection, the expression levels of CENP-F were analyzed by q-PCR and Western-blot;the proliferation of cell line U251 was explored by MTT. Results: In group Si- CENPF, the CENP-F mRNA and protein expression was significantly down- regulated (P < 0.05), and the proliferation of cell line U251 was inhibited vs blank control group and negative control group. Conclusion: Down- regulation of CENP-F by RNAi significantly inhibits the proliferation of U251 glioma cell line.