实用医学杂志
實用醫學雜誌
실용의학잡지
The Journal of Practical Medicine
2015年
17期
2883-2886
,共4页
右美托咪定%中性粒细胞%凋亡%呼吸爆发
右美託咪定%中性粒細胞%凋亡%呼吸爆髮
우미탁미정%중성립세포%조망%호흡폭발
Dexmedetomidine%Polymorphonuclear neutrophils%Apoptosis%Respiratory burst
目的：观察右美托咪定（Dex）对离体培养的人中性粒细胞（PMN）凋亡率和呼吸爆发功能的影响。方法：取健康人外周静脉血，血浆－Percoll不连续密度梯度离心法分离出PMN，分为对照组，1 ng／mL Dex组，10 ng／mL Dex组和100 ng／mL Dex组，离体培养24 h ，取培养2 h和24 h的 PMN 悬液，检测 caspase-3活性、PMN 凋亡率和呼吸爆发功能。结果：与对照组比较3种不同浓度的Dex与PMN孵育2 h，PMN的caspase-3活性、PMN凋亡率和呼吸爆发功能没有统计学差异；孵育24 h，与对照组比较，1 ng／mL Dex组PMN caspase-3活性、PMN凋亡率和呼吸爆发功能没有差异，而10 ng／mL Dex 组和100 ng／mL Dex 组PMN caspase-3活性增强、凋亡率增加和呼吸爆发功能下降；与10 ng／mL De组比较，100 ng／mL Dex组PMN caspase-3活性增强、凋亡率增加和呼吸爆发功能下降。结论：临床常用剂量的Dex（血药浓度＜1 ng／mL）不影响PMN的凋亡率和呼吸爆发功能，不影响机体的抗感染能力；而大剂量的Dex与PMN孵育一定时间（＞24 h）可促进PMN凋亡，降低其呼吸爆发功能。
目的：觀察右美託咪定（Dex）對離體培養的人中性粒細胞（PMN）凋亡率和呼吸爆髮功能的影響。方法：取健康人外週靜脈血，血漿－Percoll不連續密度梯度離心法分離齣PMN，分為對照組，1 ng／mL Dex組，10 ng／mL Dex組和100 ng／mL Dex組，離體培養24 h ，取培養2 h和24 h的 PMN 懸液，檢測 caspase-3活性、PMN 凋亡率和呼吸爆髮功能。結果：與對照組比較3種不同濃度的Dex與PMN孵育2 h，PMN的caspase-3活性、PMN凋亡率和呼吸爆髮功能沒有統計學差異；孵育24 h，與對照組比較，1 ng／mL Dex組PMN caspase-3活性、PMN凋亡率和呼吸爆髮功能沒有差異，而10 ng／mL Dex 組和100 ng／mL Dex 組PMN caspase-3活性增彊、凋亡率增加和呼吸爆髮功能下降；與10 ng／mL De組比較，100 ng／mL Dex組PMN caspase-3活性增彊、凋亡率增加和呼吸爆髮功能下降。結論：臨床常用劑量的Dex（血藥濃度＜1 ng／mL）不影響PMN的凋亡率和呼吸爆髮功能，不影響機體的抗感染能力；而大劑量的Dex與PMN孵育一定時間（＞24 h）可促進PMN凋亡，降低其呼吸爆髮功能。
목적：관찰우미탁미정（Dex）대리체배양적인중성립세포（PMN）조망솔화호흡폭발공능적영향。방법：취건강인외주정맥혈，혈장－Percoll불련속밀도제도리심법분리출PMN，분위대조조，1 ng／mL Dex조，10 ng／mL Dex조화100 ng／mL Dex조，리체배양24 h ，취배양2 h화24 h적 PMN 현액，검측 caspase-3활성、PMN 조망솔화호흡폭발공능。결과：여대조조비교3충불동농도적Dex여PMN부육2 h，PMN적caspase-3활성、PMN조망솔화호흡폭발공능몰유통계학차이；부육24 h，여대조조비교，1 ng／mL Dex조PMN caspase-3활성、PMN조망솔화호흡폭발공능몰유차이，이10 ng／mL Dex 조화100 ng／mL Dex 조PMN caspase-3활성증강、조망솔증가화호흡폭발공능하강；여10 ng／mL De조비교，100 ng／mL Dex조PMN caspase-3활성증강、조망솔증가화호흡폭발공능하강。결론：림상상용제량적Dex（혈약농도＜1 ng／mL）불영향PMN적조망솔화호흡폭발공능，불영향궤체적항감염능력；이대제량적Dex여PMN부육일정시간（＞24 h）가촉진PMN조망，강저기호흡폭발공능。
Objective To investigate the effect of dexmedetomidine (Dex) on the apoptosis and respiratory burst of human polymorphonuclear neutrophils (PMN) in vitro. Methods The peripheral venous blood was collected from healthy volunteers. Isolation of PMN was performed by using the discontinuous plasma-Percoll gradient technique. PMN was randomLy divided into four groups: control group and three different concentrations of Dex (1 ng/mL,10 ng/mL,100 ng/mL) groups. PMN was cultured in enriched RPMI-1640 media at 2 × 106/mL for 24 h. The caspase-3 activity, apoptosis rate and respiratory burst of PMN were detected at 2 and 24 h. Results Compared with the control group, the PMN which was treated with three different concentrations of Dex showed no significant difference in caspase-3 activity, apoptosis rate and respiratory burst of PMN after 2 h of incubation. Compared with the control group, 1 ng/mL concentration of Dex did not affect the caspase-3 activity, apoptosis and respiratory burst of PMN cultured for 24 h. However, 10 ng/mL and 100 ng/mL concentrations of Dex promoted the caspase-3 activity, increased apoptosis rate and reduced the respiratory burst of PMN after 24 h of incubation. Compared with 10 ng/mL Dex group, 100 ng/mL concentrations of Dex promoted the caspase-3 activity, increased apoptosis rate and reduced the respiratory burst of PMN after 24 h of incutation. Conclusion Clinically relevant concentration of Dex does not affect apoptosis and respiratory burst of PMN, while high concentration of dexmedetomidine can induce apoptosis of PMN.
