CAJ | 학술논문

目的：检测脂多糖（LPS）处理A549细胞前后发生显著变化的miRNAs，并验证miR-1247-3P在不同浓度LPS处理后细胞中表达量的变化，并探讨其可能机制。方法：对照组是以培养液处理A549细胞，实验组分别以不同浓度的LPS处理A549细胞。通过免疫细胞化学染色法、RT-PCR方法检测各组细胞中的SP-A、SP-C的表达量。用基因芯片检测细胞处理前后的表达谱，找出其中的关键miRNA，PCR的方法检测各组细胞中miRNA的表达量。结果：实验组中各组细胞的SP-A、SP-C的表达量较对照组均下降（P ＜0．05）。miRNAs芯片检测结果显示：表达显著上调的有31个，显著下调的有3个。实时荧光定量PCR测出各实验组miRNA-1247-3P的相对表达量较对照组均上升（P ＜0．05）。结论：miRNA-1247-3P在实验组有高表达，提示其可能在ARDS的发生发展过程中发挥作用。
목적：검측지다당（LPS）처리A549세포전후발생현저변화적miRNAs，병험증miR-1247-3P재불동농도LPS처리후세포중표체량적변화，병탐토기가능궤제。방법：대조조시이배양액처리A549세포，실험조분별이불동농도적LPS처리A549세포。통과면역세포화학염색법、RT-PCR방법검측각조세포중적SP-A、SP-C적표체량。용기인심편검측세포처리전후적표체보，조출기중적관건miRNA，PCR적방법검측각조세포중miRNA적표체량。결과：실험조중각조세포적SP-A、SP-C적표체량교대조조균하강（P ＜0．05）。miRNAs심편검측결과현시：표체현저상조적유31개，현저하조적유3개。실시형광정량PCR측출각실험조miRNA-1247-3P적상대표체량교대조조균상승（P ＜0．05）。결론：miRNA-1247-3P재실험조유고표체，제시기가능재ARDS적발생발전과정중발휘작용。
Objective To detect the expression pattern of microRNA in A549 cells treated by lipopolysaccharide, study the expression of miRNA-1247-3P in A549 cells under LPS treatment and explore the possible mechanism of miRNA-1247-3P in A549 cells under LPS treatment. Methods A549 cells were divided into experimental and control groups. Immunocytochemical method and RT-PCR were used to detect the changes of SP-A and SP-C. The expression of miRNAs were detected using miRNAs array in different groups. The key miR-1247-3P was collected to detect the changes of miR-1247-3P in all groups using quantitative real-time polymerase chain reaction. Results Compared with control group, the expressions of SP-A and SP-C were significantly decreased in the experimental groups (P < 0.05). MiRNA array showed that 31 miRNAs were up-regulated and 3 miRNAs were down-regulated. Compared with control group, the expression of miR-1247-3P was significantly increased in the experimental groups (P < 0.05). Conclusion The increased expression of miR-1247-3P may play an important role in the pathogenesis of ARDS.