山东农业大学学报(自然科学版)
山東農業大學學報(自然科學版)
산동농업대학학보(자연과학판)
Journal of Shandong Agricultural University (Natural Science Edition)
2015年
5期
676-681
,共6页
顾地周%杨丽娟%王曼宁%刘宇%赵明明%沈红梅%朱俊义%周繇
顧地週%楊麗娟%王曼寧%劉宇%趙明明%瀋紅梅%硃俊義%週繇
고지주%양려연%왕만저%류우%조명명%침홍매%주준의%주요
牛皮杜鹃%嫩茎%均匀设计法%植株再生
牛皮杜鵑%嫩莖%均勻設計法%植株再生
우피두견%눈경%균균설계법%식주재생
Rhododendron chrysanthum Pall.%tender stem segments%uniform design method%plantlet regeneration
本研究以牛皮杜鹃嫩茎段为外植体,通过均匀设计法对影响牛皮杜鹃腋芽萌发生长同时生根的各主要因素及其水平的作用进行了实验探讨,旨在探索一种试管内牛皮杜鹃高效植株的再生方法。结果表明,最适合牛皮杜鹃嫩茎段生根同时腋芽萌发生长的培养基为:基本培养基+KT 0.13~0.14 mg·L-1+IBA 0.05~0.08 mg·L-1+NAA 0.02~0.05 mg·L-1+GA32.70~2.90 mg·L-1,植株再生率为99.2%。以再生植株茎节为材料进行快繁的结果表明,在28 d的培养周期内,每段平均增殖6倍以上。再生植株的移栽成活率达96.0%以上。成功建立了高效植株再生体系,所建立的高效植株再生体系适合于牛皮杜鹃种苗工厂化生产。
本研究以牛皮杜鵑嫩莖段為外植體,通過均勻設計法對影響牛皮杜鵑腋芽萌髮生長同時生根的各主要因素及其水平的作用進行瞭實驗探討,旨在探索一種試管內牛皮杜鵑高效植株的再生方法。結果錶明,最適閤牛皮杜鵑嫩莖段生根同時腋芽萌髮生長的培養基為:基本培養基+KT 0.13~0.14 mg·L-1+IBA 0.05~0.08 mg·L-1+NAA 0.02~0.05 mg·L-1+GA32.70~2.90 mg·L-1,植株再生率為99.2%。以再生植株莖節為材料進行快繁的結果錶明,在28 d的培養週期內,每段平均增殖6倍以上。再生植株的移栽成活率達96.0%以上。成功建立瞭高效植株再生體繫,所建立的高效植株再生體繫適閤于牛皮杜鵑種苗工廠化生產。
본연구이우피두견눈경단위외식체,통과균균설계법대영향우피두견액아맹발생장동시생근적각주요인소급기수평적작용진행료실험탐토,지재탐색일충시관내우피두견고효식주적재생방법。결과표명,최괄합우피두견눈경단생근동시액아맹발생장적배양기위:기본배양기+KT 0.13~0.14 mg·L-1+IBA 0.05~0.08 mg·L-1+NAA 0.02~0.05 mg·L-1+GA32.70~2.90 mg·L-1,식주재생솔위99.2%。이재생식주경절위재료진행쾌번적결과표명,재28 d적배양주기내,매단평균증식6배이상。재생식주적이재성활솔체96.0%이상。성공건립료고효식주재생체계,소건립적고효식주재생체계괄합우우피두견충묘공엄화생산。
In this study, the young stems of Rhododendron chrysanthum were used as explants, the main factors with different levels of axillary sprouting, shoots growth and shoots rooting were investigated through uniform design experiments. To explore a method of efficient plantlet regeneration in vitro. The results showed that the basic medium+KT 0.13~0.14 mg·L-1+IBA 0.05~0.08 mg·L-1+NAA 0.02~0.05 mg·L-1+GA3 2.70~2.90 mg·L-1 was the most suitable for axillary sprouting, shoots growth and shoots rooting, the rate of plantlet regeneration was 99.2%. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 6-fold proliferation rate was achieved within 28 days. The survival rate of regenerated plants was more than 96.0%. Efficient plantlet regeneration system of Rhododendron chrysanthum have been successfully established, which is suitable to industrialized seedling for Rhododendron chrysanthum.