中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
36期
5769-5773
,共5页
干细胞%骨髓干细胞%膀胱脱细胞基质%支架材料%骨髓间充质干细胞%膀胱%脱细胞技术%生物相容性
榦細胞%骨髓榦細胞%膀胱脫細胞基質%支架材料%骨髓間充質榦細胞%膀胱%脫細胞技術%生物相容性
간세포%골수간세포%방광탈세포기질%지가재료%골수간충질간세포%방광%탈세포기술%생물상용성
背景：国内外许多研究者一直寻找理想的种子细胞与合适的支架材料复合，试图模拟接近正常生理功能的组织工程化尿路替代物。<br> 目的：探讨骨髓间充质干细胞与兔膀胱脱细胞基质支架的生物相容性。<br> 方法：采用密度梯度离心法分离培养兔骨髓间充质干细胞，将第3代兔骨髓间充质干细胞接种到兔膀胱脱细胞基质上进行复合培养，每天进行细胞计数，连续12 d，绘制细胞生长曲线，以单独培养骨髓间充质干细胞为对照组。<br> 结果与结论：骨髓间充质干细胞成功种植到膀胱脱细胞基质上，倒置显微镜下可见骨髓间充质干细胞从膀胱脱细胞基质边缘爬出，膀胱脱细胞基质周围有大量长梭形骨髓间充质干细胞生长。接种5d内，两组细胞均呈现出平缓生长的状态，接种6-9 d，生长曲线逐渐变得陡峭，细胞呈倍数状态进行分裂生长，速度较快，接种10-12 d，再次趋于平缓状态。两组细胞生长曲线基本重合，可推断兔骨髓间充质干细胞与膀胱脱细胞基质生物相容性良好。
揹景：國內外許多研究者一直尋找理想的種子細胞與閤適的支架材料複閤，試圖模擬接近正常生理功能的組織工程化尿路替代物。<br> 目的：探討骨髓間充質榦細胞與兔膀胱脫細胞基質支架的生物相容性。<br> 方法：採用密度梯度離心法分離培養兔骨髓間充質榦細胞，將第3代兔骨髓間充質榦細胞接種到兔膀胱脫細胞基質上進行複閤培養，每天進行細胞計數，連續12 d，繪製細胞生長麯線，以單獨培養骨髓間充質榦細胞為對照組。<br> 結果與結論：骨髓間充質榦細胞成功種植到膀胱脫細胞基質上，倒置顯微鏡下可見骨髓間充質榦細胞從膀胱脫細胞基質邊緣爬齣，膀胱脫細胞基質週圍有大量長梭形骨髓間充質榦細胞生長。接種5d內，兩組細胞均呈現齣平緩生長的狀態，接種6-9 d，生長麯線逐漸變得陡峭，細胞呈倍數狀態進行分裂生長，速度較快，接種10-12 d，再次趨于平緩狀態。兩組細胞生長麯線基本重閤，可推斷兔骨髓間充質榦細胞與膀胱脫細胞基質生物相容性良好。
배경：국내외허다연구자일직심조이상적충자세포여합괄적지가재료복합，시도모의접근정상생리공능적조직공정화뇨로체대물。<br> 목적：탐토골수간충질간세포여토방광탈세포기질지가적생물상용성。<br> 방법：채용밀도제도리심법분리배양토골수간충질간세포，장제3대토골수간충질간세포접충도토방광탈세포기질상진행복합배양，매천진행세포계수，련속12 d，회제세포생장곡선，이단독배양골수간충질간세포위대조조。<br> 결과여결론：골수간충질간세포성공충식도방광탈세포기질상，도치현미경하가견골수간충질간세포종방광탈세포기질변연파출，방광탈세포기질주위유대량장사형골수간충질간세포생장。접충5d내，량조세포균정현출평완생장적상태，접충6-9 d，생장곡선축점변득두초，세포정배수상태진행분렬생장，속도교쾌，접충10-12 d，재차추우평완상태。량조세포생장곡선기본중합，가추단토골수간충질간세포여방광탈세포기질생물상용성량호。
BACKGROUND:In the repair of urinary tract defects, we have been actively trying to construct the urinary tract substitutes with normal physiological function through combining ideal seed cel s and proper scaffold materials by tissue engineering method. OBJECTIVE:To investigate the biocompatibility of bone marrow mesenchymal stem cel s with rabbit bladder acel ular matrix scaffold. METHODS:Rabbit bone marrow mesenchymal stem cel s were isolated and cultured using density gradient centrifugation method. Passage 3 rabbit bone marrow mesenchymal stem cel s were cultured on the rabbit bladder acel ular matrix. The cel s were counted every day for 12 days, to drawn a cel growth curve. Bone marrow mesenchymal stem cel s cultured alone were used as control group. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s were successful y seeded onto the bladder acel ular matrix. Under the inverted microscope, the cel s grew out of the bladder acel ular matrix, and a great amount of long spindle-shaped cel s were found around the bladder acel ular matrix. With 5 days of inoculation, the cel s in the two groups grew gently;at 6-9 days, the cel growth curve gradual y became steeper, and the cel division and growth were increased exponential y;at 10-12 days, the cel s recovered to a gentle state. Cel growth curves in the two groups were basical y coincident, suggesting that rabbit bone marrow mesenchymal stem cel s have good biocompatibility with the bladder matrix.
