CAJ | 학술논문

背景：医学界普遍认为支气管哮喘是一种与Th2相关的免疫反应疾病，目前尚缺乏理想的治疗方法。骨髓间充质干细胞作为一种成体干细胞，不仅具有增殖和多向分化能力，还具有低免疫原性和免疫调节能力。 　　目的：探讨骨髓间充质干细胞培养上清液对支气管哮喘小鼠肺部炎症的影响。 　　方法：将20只实验小鼠随机分为对照组和实验组，每组10只，在第0天及第14天小鼠腹腔注射卵清蛋白溶液致敏，并在第24-26天雾化吸入卵清蛋白溶液激发。实验组从第24天开始，在每次激发前2 h，腹腔注射制备好的骨髓间充质干细胞培养上清液2 mL，对照组腹腔注射生理盐水。末次激发后麻醉致小鼠安乐死亡，采取血清、肺泡灌洗液及肺脏组织进行研究。 　　结果与结论：①对照组小鼠肺组织结构发生异常，黏膜下层和肌层内能够看见大量嗜酸性粒细胞及单核细胞浸润，经骨髓间充质干细胞培养液治疗后，实验组小鼠肺部炎症较轻。②实验组白细胞总数、嗜酸性粒细胞数、嗜酸性粒细胞百分比显著低于对照组(P <0.01)。③实验组肺泡灌洗液以及血清中白细胞介素17水平显著低于对照组(P <0.05)；肺泡灌洗液以及血清中白细胞介素4水平与对照组比较差异无显著性意义(P >0.05)。上述结果提示支气管哮喘小鼠腹腔注入骨髓间充质干细胞培养上清液能够减轻肺部炎性病理反应严重程度，降低肺泡灌洗液以及血清中相关的炎症指标水平。
배경：의학계보편인위지기관효천시일충여Th2상관적면역반응질병，목전상결핍이상적치료방법。골수간충질간세포작위일충성체간세포，불부구유증식화다향분화능력，환구유저면역원성화면역조절능력。 　　목적：탐토골수간충질간세포배양상청액대지기관효천소서폐부염증적영향。 　　방법：장20지실험소서수궤분위대조조화실험조，매조10지，재제0천급제14천소서복강주사란청단백용액치민，병재제24-26천무화흡입란청단백용액격발。실험조종제24천개시，재매차격발전2 h，복강주사제비호적골수간충질간세포배양상청액2 mL，대조조복강주사생리염수。말차격발후마취치소서안악사망，채취혈청、폐포관세액급폐장조직진행연구。 　　결과여결론：①대조조소서폐조직결구발생이상，점막하층화기층내능구간견대량기산성립세포급단핵세포침윤，경골수간충질간세포배양액치료후，실험조소서폐부염증교경。②실험조백세포총수、기산성립세포수、기산성립세포백분비현저저우대조조(P <0.01)。③실험조폐포관세액이급혈청중백세포개소17수평현저저우대조조(P <0.05)；폐포관세액이급혈청중백세포개소4수평여대조조비교차이무현저성의의(P >0.05)。상술결과제시지기관효천소서복강주입골수간충질간세포배양상청액능구감경폐부염성병리반응엄중정도，강저폐포관세액이급혈청중상관적염증지표수평。
BACKGROUND:Bronchial asthma is considered general y to have an association with Th2 immune response disease, but there is no ideal treatment. Bone marrow mesenchymal stem cel s as a kind of adult stem cel s not only have the multipotent differentiation and proliferation capacity, but also have low immunogenicity and immunoregulation ability. OBJECTIVE:To investigate the effect of culture supernatant of bone marrow mesenchymal stem cel s on lung inflammation of bronchial asthma mice. METHODS:Twenty experimental mice were randomly divided into control and experimental groups, 10 mice in each group. At 0 and 14 days, intraperitoneal injection of ovalbumin induced sensitization in mice, and at 24-26 days, aerosolized ovalbumin solution was used for excitation. From the 24th day, in the experimental group, bone marrow mesenchymal stem cel supernatant was intraperitoneal y injected at 2 hours before excitation;meanwhile, normal saline was injected in the control group. At the last of excitation, the mice were sacrificed under anesthesia to take serum samples, bronchoalveolar lavage fluid and lung tissues. RESULTS AND CONCLUSION:(1) Mice in the control group appeared to have abnormal lung tissue structure, and there were a large amount of eosinophils and monocytes in the submucosa and muscularis. However, lung inflammation was relieved in the experimental group after bone marrow mesenchymal stem cel treatment. (2) The levels of interleukin-17 in the bronchoalveolar lavage fluid and serum were significantly lower in the experimental group than the control group (P<0.05), but there was no difference in the levels of interleukin-4 between the two groups (P>0.05). These findings indicate that the intraperitoneal injection of bone marrow mesenchymal stem cel s can ease lung inflammation and reduce levels of inflammatory markers in the bronchoalveolar lavage fluid and serum of bronchial asthma mice.