山东农业大学学报（自然科学版）
山東農業大學學報（自然科學版）
산동농업대학학보（자연과학판）
Journal of Shandong Agricultural University (Natural Science Edition)
2015年
5期
648-651
,共4页
依诺沙星%铕%荧光光谱法%肝素
依諾沙星%銪%熒光光譜法%肝素
의낙사성%유%형광광보법%간소
Enoxacin%Europium%Spectrofluorimetric%Heparin
在pH值为7.40的HMT-HCl缓冲溶液中，铕（Eu3+）能和依诺沙星（Enoxacin）形成络合物，发出Eu3+的特征荧光，最大激发和发射波长分别为330 nm和612 nm，狭缝均为5 nm，肝素的加入能使Eu3+的特征荧光显著增强。在线性范围0.117~5.85μg·mL-1内，肝素的浓度和荧光强度成很好的线性关系，线性方程为△F=-5.691+6.98 c （r=0.994）（c：μg·mL-1）；检出限为0.06μg·mL-1。本方法用于实际样品的测定，灵敏度高、选择性好、干扰少、操作简单，结果令人满意。
在pH值為7.40的HMT-HCl緩遲溶液中，銪（Eu3+）能和依諾沙星（Enoxacin）形成絡閤物，髮齣Eu3+的特徵熒光，最大激髮和髮射波長分彆為330 nm和612 nm，狹縫均為5 nm，肝素的加入能使Eu3+的特徵熒光顯著增彊。在線性範圍0.117~5.85μg·mL-1內，肝素的濃度和熒光彊度成很好的線性關繫，線性方程為△F=-5.691+6.98 c （r=0.994）（c：μg·mL-1）；檢齣限為0.06μg·mL-1。本方法用于實際樣品的測定，靈敏度高、選擇性好、榦擾少、操作簡單，結果令人滿意。
재pH치위7.40적HMT-HCl완충용액중，유（Eu3+）능화의낙사성（Enoxacin）형성락합물，발출Eu3+적특정형광，최대격발화발사파장분별위330 nm화612 nm，협봉균위5 nm，간소적가입능사Eu3+적특정형광현저증강。재선성범위0.117~5.85μg·mL-1내，간소적농도화형광강도성흔호적선성관계，선성방정위△F=-5.691+6.98 c （r=0.994）（c：μg·mL-1）；검출한위0.06μg·mL-1。본방법용우실제양품적측정，령민도고、선택성호、간우소、조작간단，결과령인만의。
A new method for determination of Heparin by fluorometry method was established. In HMT-HCl buffer solution at pH 7.40, Eu3+formed complex with enoxacin, which emitted characteristic fluorescence of Eu3+with maximum excitation wavelength at 330 nm, maximum emission wavelength at 612 nm and slit width at 5 nm. Additional Heparin resulted in a remarkable increase in the Eu3+ characteristic fluorescence. Within the range of 0.117~5.85 μg·mL-1, the concentration of Heparin had a linear relationship with the fluorescent intensity, with linear equation of△F=-5.691+6.98 c(r=0.994)(c:μg·mL-1)and detection limit of 0.06μg·mL-1. This method generated high selectivity, high sensitivity and low disturbance, which had been applied to the determination of practical samples with satisfactory result.
