CAJ | 학술논문

目的探讨骨髓间充质干细胞(MSC)预处理脑死亡(BD)供鼠对移植肾的保护作用.方法实验分正常移植组、脑死亡供肾移植组(BD移植组)和输注MSC+脑死亡供肾移植组(MSC处理组),雄性F344大鼠为供者,雄性Lewis大鼠为受者,正常移植组为常规移植;BD移植组为脑死亡F344大鼠供肾;MSC处理组在移植前输注预先制备的来自F344大鼠的MSC,然后接受脑死亡F344大鼠供肾移植.移植后给予环孢素A,连用10天.术后第10天切除其右肾.测定术后第14、21、28和35天血肌酐水平.术后35 d获取移植肾标本,行病理学观察;免疫组化检测移植肾组织中白细胞介素(IL)-1β和肿瘤坏死因子α(TNF-α)表达情况.结果 BD移植组术后各检测时点的血肌酐水平均高于其余两组(P＜0.05),而MSC处理组和正常移植组间除第21天的血清肌酐值外,其余时间段的差异没有统计学意义.BD移植组移植肾组织中可见单核细胞浸润,其肾小管上皮炎症明显重于其他两组,MSC处理组与正常移植组的差异不大.BD移植组移植肾肾小球上皮细胞、肾小管上皮细胞、间质细胞的IL-1β和TNF-α的表达均呈阳性,染色程度较强,明显强于其他两组(H=7.210,P=0.027),而MSC处理组和正常移植组间的差异无统计学意义.结论MSC预处理脑死亡供鼠,可能通过减少移植肾炎症细胞浸润,降低TNF-α和IL-1β表达来减轻移植肾损伤.
목적 탐토골수간충질간세포(MSC)예처리뇌사망(BD)공서대이식신적보호작용.방법 실험분정상이식조、뇌사망공신이식조(BD이식조)화수주MSC+뇌사망공신이식조(MSC처리조),웅성F344대서위공자,웅성Lewis대서위수자,정상이식조위상규이식;BD이식조위뇌사망F344대서공신;MSC처리조재이식전수주예선제비적래자F344대서적MSC,연후접수뇌사망F344대서공신이식.이식후급여배포소A,련용10천.술후제10천절제기우신.측정술후제14、21、28화35천혈기항수평.술후35 d획취이식신표본,행병이학관찰;면역조화검측이식신조직중백세포개소(IL)-1β화종류배사인자α(TNF-α)표체정황.결과 BD이식조술후각검측시점적혈기항수평균고우기여량조(P＜0.05),이MSC처리조화정상이식조간제제21천적혈청기항치외,기여시간단적차이몰유통계학의의.BD이식조이식신조직중가견단핵세포침윤,기신소관상피염증명현중우기타량조,MSC처리조여정상이식조적차이불대.BD이식조이식신신소구상피세포、신소관상피세포、간질세포적IL-1β화TNF-α적표체균정양성,염색정도교강,명현강우기타량조(H=7.210,P=0.027),이MSC처리조화정상이식조간적차이무통계학의의.결론MSC예처리뇌사망공서,가능통과감소이식신염증세포침윤,강저TNF-α화IL-1β표체래감경이식신손상.
Objective To investigate the protective effects on the renal allografts from brain dead (BD) donor rats pretreated with bone marrow mesenchymal stem cells (MSCs).Method Three groups [normal transplant group (G1).BD transplant group (G2),and MSCs pretreated + BD transplant group (G3)] were set up.Male F344 rats served as donors and male Lewis rats as recipients.In G1,kidneys from F344 donor rats were implanted into Lewis recipients.In G2,kidneys from F344 BD donor rats were engrafted into Lewis recipients.In G3,after BD was established in F344 rats,MSCs were given intravenously to the rats.The kidneys harvested 6 h later were transplanted to Lewis recipients.Cyclosporine was intromuscularly given daily to the recipient rats for 10 days.Right kidneys were resected from recipients on day 10.Creatinine level was examined on day 14,21,28,and 35.Renal allografts harvested on day 35 were pathologically detected.The irnmunochemistry expression of interleukin (IL)-1β and tumor necrotic factor (TNF)-α in renal allograft tissue was tested.Result Serum creatinine levels in G2 were remarkably higher than those in G1 and G3 (P＜0.01) on day 14,21,28,and 35 postoperatively.The creatinine levels on the above mentioned time points had no statistically significant difference between G3 and G1 except on day 21.Postoperative pathological changes in G2 of both pronounced infiltration of mononuclear cells and tubular epithelia[inflammation were notably increased in renal allografts as compared with those in G1 and G3.There was no obvious difference between G1 and G3 in infiltrated mononuclear cells and tubular epithelial inflammation.Positive expression levels of both IL-1β and TNF-α in glomerular,tubular and interstitial epithelial cells were statistically enhanced in G2 as compared with those in G1 and G3 (H =7.210,P =0.027),while there was no statistically significant difference in the expression of both IL-1[β and TNF-α between G1 and G3.Conclusion Brain dead donor rats pretreated with bone marrow MSCs might reduce renal allograft injury via decreasing both inflammatory cell infiltration and IL-1β and TNF-α expression.