CAJ | 학술논문

背景：研究发现神经干细胞或者骨髓基质干细胞可脑内移植治疗中枢神经系统疾病，但是两种干细胞移植治疗效果的比较相对较少。 　　目的：观察比较中脑神经干细胞和骨髓基质干细胞对帕金森大鼠行为学及脑组织形态学的影响。 　　方法：58只SD大鼠构建帕金森病模型，随机分为3组，分别为骨髓基质干细胞组(20只)、中脑神经干细胞组(20只)、生理盐水组(18只)。造模成功后3周，选取右侧纹状体2个坐标点注入骨髓基质干细胞悬液、中脑神经干细胞悬液、生理盐水5μL。移植后5个月腹腔注射阿朴吗啡观察大鼠的行为学变化，取纹状体脑组织制备石蜡切片进行免疫组化荧光染色。 　　结果与结论：治疗后第5个月骨髓基质干细胞组和中脑神经干细胞组大鼠旋转次数较治疗前显著下降(P<0.05)，且明显低于生理盐水组(P<0.05)，骨髓基质干细胞组和神经干细胞组比较差异无显著性意义(P>0.05)。移植后第1周，骨髓基质干细胞组纹状体内有 BrdU 与 Nestin 双重染色细胞；移植后第1个月，脑纹状体内出现BrdU/GFAP双重染色细胞和BrdU/NSE双重染色细胞；纹状体内存在TH阳性细胞，但未出现明显BrdU/TH双重染色细胞；移植1个月之后，BrdU与Nestin双重染色细胞数量逐渐减少，最终基本消失，但仍然存在一定的数量的BrdU/GFAP、BrdU/NSE双重染色细胞，BrdU/GFAP阳性细胞数量相对较多。同一时间点，中脑神经干细胞组也存在类似情况，但生理盐水组未发现双标细胞。结果表明，中脑神经干细胞和骨髓基质干细胞移植均可改善帕金森大鼠的行为学，且可分化为神经元、星形胶质细胞、多巴胺能神经元。
배경：연구발현신경간세포혹자골수기질간세포가뇌내이식치료중추신경계통질병，단시량충간세포이식치료효과적비교상대교소。 　　목적：관찰비교중뇌신경간세포화골수기질간세포대파금삼대서행위학급뇌조직형태학적영향。 　　방법：58지SD대서구건파금삼병모형，수궤분위3조，분별위골수기질간세포조(20지)、중뇌신경간세포조(20지)、생리염수조(18지)。조모성공후3주，선취우측문상체2개좌표점주입골수기질간세포현액、중뇌신경간세포현액、생리염수5μL。이식후5개월복강주사아박마배관찰대서적행위학변화，취문상체뇌조직제비석사절편진행면역조화형광염색。 　　결과여결론：치료후제5개월골수기질간세포조화중뇌신경간세포조대서선전차수교치료전현저하강(P<0.05)，차명현저우생리염수조(P<0.05)，골수기질간세포조화신경간세포조비교차이무현저성의의(P>0.05)。이식후제1주，골수기질간세포조문상체내유 BrdU 여 Nestin 쌍중염색세포；이식후제1개월，뇌문상체내출현BrdU/GFAP쌍중염색세포화BrdU/NSE쌍중염색세포；문상체내존재TH양성세포，단미출현명현BrdU/TH쌍중염색세포；이식1개월지후，BrdU여Nestin쌍중염색세포수량축점감소，최종기본소실，단잉연존재일정적수량적BrdU/GFAP、BrdU/NSE쌍중염색세포，BrdU/GFAP양성세포수량상대교다。동일시간점，중뇌신경간세포조야존재유사정황，단생리염수조미발현쌍표세포。결과표명，중뇌신경간세포화골수기질간세포이식균가개선파금삼대서적행위학，차가분화위신경원、성형효질세포、다파알능신경원。
BACKGROUND:For treatment of central nervous system diseases, neural stem cel s (NSCs) or bone marrow stromal stem cel s (BMSCs) can be transplanted into the brain, but there are less reports to compare the effects of two kinds of stem cel transplantation. OBJECTIVE:To explore the effect of midbrain NSCs and BMSCs on the behavior and brain morphology of rats with Parkinson’s disease. METHODS:Fifty-eight Sprague-Dawley rats were enrol ed to establish Parkinson’s disease models, and then randomly divided into three groups, which were treated with 5μL midbrain NSCs (n=20), 5μL BMSCs (n=20) and 5μL normal saline (n=18) via two coordinate points of the right striatum at 3 weeks after modeling, respectively. At 5 months after transplantation, the rats underwent intraperitoneal injection of apomorphine to observe behavioral changes, and then, the striatum was taken for immunohistochemistry staining. RESULTS AND CONCLUSION:The number of rotations was reduced significantly in the BMSCs and midbrain NSCs groups at 5 months after transplantation (P<0.05), which was significantly lower than that in the normal saline group (P<0.05). However, there was no significant difference between the BMSCs and NSCs groups (P>0.05). In the BMSCs group, BrdU/Nestin positive cel s were seen in the brain stratium at 1 week after transplantation;BrdU/GFAP and BrdU/NSE positive cel s as wel as TH positive cel s rather than BrdU/TH positive cel s were found in the brain stratium at 1 month after transplantation;after that, the number of BrdU/Nestin positive cel s was reduced gradual y and disappeared ultimately, but there were stil a certain number of BrdU/GFAP and BrdU/NSE positive cel s, especial y the former ones. Meanwhile, the NSCs group also had a similar situation, but no double-labeled cel s were in the normal saline group. These findings indicate that midbrain NSCs and BMSCs transplantation can both improve the behavior of Parkinson’s disease rats, and differentiate into neurons, astrocytes and dopaminergic neurons.