CAJ | 학술논문

背景：1型糖尿病是一种自身免疫性疾病，以胰腺β细胞选择性破坏，导致患者体内胰岛素分泌绝对不足为特征。脐血干细胞具有多分化潜能，在体内外均可以诱导分化为胰岛细胞，发挥出一定的降糖作用。 　　目的：探讨脐血干细胞对1型糖尿病大鼠血糖水平及胰腺组织PDX-1、MafA表达的影响。 　　方法：30只SD大鼠随机分为3组，每组10只，治疗组和模型组大鼠建立1型糖尿病模型，造模成功后，治疗组尾静脉一次性注射脐血干细胞，正常组给予相同体积的生理盐水，模型组给予相同体积的脐血干细胞缓冲液。采用口服葡萄糖耐量试验评价大鼠胰岛功能，苏木精-伊红染色观察大鼠胰腺形态，Western blot 和PCR 检测胰腺组织PDX-1、MafA蛋白和mRNA表达。 　　结果与结论：①模型组和治疗组大鼠0，30，60，90 min的血糖值均显著高于正常组，差异均有显著性意义(P<0.05)；120 min时间点模型组血糖值显著高于正常组(P<0.05)；治疗组则与正常组之间差异无显著性意义(P>0.05)。②模型组胰岛数量出现下降，边界模糊不清，呈不规则形态，治疗组胰岛数量出现一定的减少，但尚维持清晰的结构。③治疗组PDX-1及MafA表达水平与正常组比较差异无显著性意义(P>0.05)，但显著高于模型组(P<0.05)。以上结果表明，脐血干细胞可以显著降低1型糖尿病大鼠的血糖水平，改善胰岛功能及胰腺组织形态，并具有上调PDX-1、MafA表达的作用。
배경：1형당뇨병시일충자신면역성질병，이이선β세포선택성파배，도치환자체내이도소분비절대불족위특정。제혈간세포구유다분화잠능，재체내외균가이유도분화위이도세포，발휘출일정적강당작용。 　　목적：탐토제혈간세포대1형당뇨병대서혈당수평급이선조직PDX-1、MafA표체적영향。 　　방법：30지SD대서수궤분위3조，매조10지，치료조화모형조대서건립1형당뇨병모형，조모성공후，치료조미정맥일차성주사제혈간세포，정상조급여상동체적적생리염수，모형조급여상동체적적제혈간세포완충액。채용구복포도당내량시험평개대서이도공능，소목정-이홍염색관찰대서이선형태，Western blot 화PCR 검측이선조직PDX-1、MafA단백화mRNA표체。 　　결과여결론：①모형조화치료조대서0，30，60，90 min적혈당치균현저고우정상조，차이균유현저성의의(P<0.05)；120 min시간점모형조혈당치현저고우정상조(P<0.05)；치료조칙여정상조지간차이무현저성의의(P>0.05)。②모형조이도수량출현하강，변계모호불청，정불규칙형태，치료조이도수량출현일정적감소，단상유지청석적결구。③치료조PDX-1급MafA표체수평여정상조비교차이무현저성의의(P>0.05)，단현저고우모형조(P<0.05)。이상결과표명，제혈간세포가이현저강저1형당뇨병대서적혈당수평，개선이도공능급이선조직형태，병구유상조PDX-1、MafA표체적작용。
BACKGROUND:Type 1 diabetes mel itus is an autoimmune disease, which is characterized as the selective destruction of pancreatic beta cel s in the body, resulting in the lack of insulin secretion. Umbilical cord blood stem cel s have the potential to differentiate into islet cel s in vitro and in vivo, which play a certain hypoglycemic effect. OBJECTIVE:To explore the effects of umbilical cord blood stem cel s on blood glucose levels and PDX-1 and MafA levels in the pancreatic tissue of type 1 diabetic rats. METHODS:Thirty Sprague-Dawley rats were randomly divided into three groups, with 10 rats in each group. In treatment and model groups, type 1 diabetes mel itus modes were established. After modeling, the treatment group was given a single tail vein injection of umbilical cord blood stem cel s;the normal control group was given the same volume of normal saline;the model group was given the same volume of umbilical cord blood stem cel buffer solution. Oral glucose tolerance test was adopted to assess the islet function of rats;hematoxylin-eosin staining was used to observe the pancreatic morphology of rats;western blot and PCR methods were employed to detect expressions of PDX-1 and MafA in pancreatic tissues at protein and mRNA levels. RESULTS AND CONCLUSION:(1) Compared with the normal control group, the levels of blood glucose in the model and treatment groups were significantly higher at 0, 30, 60, 90 minutes (P<0.05). At 120 minutes, the blood glucose level in the model group was significantly higher than that in the normal control group (P<0.05), but there was no difference between the treatment and normal control groups (P>0.05). (2) The number of islets in the model group was decreased, and the boundary was unclear and irregular;in the treatment group, the number of islets was decreased, but the boundary was stil clear. (3) The expressions of PDX-1 and MafA in the treatment group were similar to those in the normal control group (P>0.05), but significantly higher than those in the model group (P<0.05). These findings suggest that the umbilical cord blood stem cel transplantation can significantly reduce the blood glucose levels in type 1 diabetic rats, improve the function of islet and morphology of pancreas, and up-reuglate the expressions of PDX-1 and MafA.