中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
36期
5872-5877
,共6页
干细胞%移植%心肌细胞%定向分化%诱导剂%干细胞动员%运动:心肌梗死
榦細胞%移植%心肌細胞%定嚮分化%誘導劑%榦細胞動員%運動:心肌梗死
간세포%이식%심기세포%정향분화%유도제%간세포동원%운동:심기경사
背景：干细胞移植治疗心血管疾病表现出传统方法无可比拟的优越性，但干细胞向心肌细胞自发分化的效率非常低，而且影响干细胞分化的因素很多。<br> 目的：阐明不同来源干细胞治疗心肌梗死的优缺点，探讨提高心肌细胞分化效率的方法，最佳诱导分化条件及运动促进干细胞动员、诱导内源性心肌细胞再生的机制。<br> 方法：由第一作者检索1985至2015年PubMed数据库和中国知网收录的与干细胞治疗心肌梗死、干细胞向心肌细胞分化运动影响干细胞增殖及心肌细胞再生的相关文献。英文检索词为“stem cel s，myocardial infarction，myocardial regeneration，cardiac cel ，exercise”，中文检索词为“干细胞，心肌梗死，心肌再生，心肌细胞，运动”，共纳入54篇文章进行综述。<br> 结果与结论：为了提高心肌细胞分化率，近年来多种化学诱导剂和生物成分先后应用于干细胞向心肌细胞分化的研究，模拟心肌微环境和血管细胞生长因子的作用也是诱导心肌细胞分化的主要方法。通过有氧运动促进干细胞动员来诱导缺血心脏血管新生和上调各种血管生长因子表达，可促进心肌细胞增殖和修复。有关干细胞的获取、移植排斥反应、向心肌细胞定向分化的调控机制还需进行深入探讨。
揹景：榦細胞移植治療心血管疾病錶現齣傳統方法無可比擬的優越性，但榦細胞嚮心肌細胞自髮分化的效率非常低，而且影響榦細胞分化的因素很多。<br> 目的：闡明不同來源榦細胞治療心肌梗死的優缺點，探討提高心肌細胞分化效率的方法，最佳誘導分化條件及運動促進榦細胞動員、誘導內源性心肌細胞再生的機製。<br> 方法：由第一作者檢索1985至2015年PubMed數據庫和中國知網收錄的與榦細胞治療心肌梗死、榦細胞嚮心肌細胞分化運動影響榦細胞增殖及心肌細胞再生的相關文獻。英文檢索詞為“stem cel s，myocardial infarction，myocardial regeneration，cardiac cel ，exercise”，中文檢索詞為“榦細胞，心肌梗死，心肌再生，心肌細胞，運動”，共納入54篇文章進行綜述。<br> 結果與結論：為瞭提高心肌細胞分化率，近年來多種化學誘導劑和生物成分先後應用于榦細胞嚮心肌細胞分化的研究，模擬心肌微環境和血管細胞生長因子的作用也是誘導心肌細胞分化的主要方法。通過有氧運動促進榦細胞動員來誘導缺血心髒血管新生和上調各種血管生長因子錶達，可促進心肌細胞增殖和脩複。有關榦細胞的穫取、移植排斥反應、嚮心肌細胞定嚮分化的調控機製還需進行深入探討。
배경：간세포이식치료심혈관질병표현출전통방법무가비의적우월성，단간세포향심기세포자발분화적효솔비상저，이차영향간세포분화적인소흔다。<br> 목적：천명불동래원간세포치료심기경사적우결점，탐토제고심기세포분화효솔적방법，최가유도분화조건급운동촉진간세포동원、유도내원성심기세포재생적궤제。<br> 방법：유제일작자검색1985지2015년PubMed수거고화중국지망수록적여간세포치료심기경사、간세포향심기세포분화운동영향간세포증식급심기세포재생적상관문헌。영문검색사위“stem cel s，myocardial infarction，myocardial regeneration，cardiac cel ，exercise”，중문검색사위“간세포，심기경사，심기재생，심기세포，운동”，공납입54편문장진행종술。<br> 결과여결론：위료제고심기세포분화솔，근년래다충화학유도제화생물성분선후응용우간세포향심기세포분화적연구，모의심기미배경화혈관세포생장인자적작용야시유도심기세포분화적주요방법。통과유양운동촉진간세포동원래유도결혈심장혈관신생화상조각충혈관생장인자표체，가촉진심기세포증식화수복。유관간세포적획취、이식배척반응、향심기세포정향분화적조공궤제환수진행심입탐토。
BACKGROUND:Stem cel transplantation has incomparable superiorities in the treatment of cardiovascular diseases. But stem cel s have a very low efficiency to differentiate into myocardial cel s spontaneously, and there are many factors influencing stem cel differentiation. OBJECTIVE:To clarify the pros and cons of different sources of stem cel s in the treatment of myocardial infarction, to investigate the methods for improving the differentiation efficiency of myocardial cel s, optimal differentiation conditions and mechanism underlying exercise-induced stem cel mobilization and endogenous myocardial cel regeneration. METHODS:A computer-based search of CNKI and PubMed databases was performed by the first author for articles related to stem cel therapy for myocardial infarction and stem cel differentiation into myocardial cel s, exercise effect on stem cel proliferation and myocardial cel regeneration published from 1985 to 2015. The key words were“stem cel s, myocardial infarction, myocardial regeneration, cardiac cel , exercise”in Chinese and English, respectively. Final y, 54 articles were included in result analysis. RESULTS AND CONCLUSION:In recent years, a variety of chemical inducers and biological components have been used in the myocardial differentiation of stem cel s. Simulation of myocardial microenvironment and vascular cel growth factors are the main methods of inducing myocardial differentiation. Aerobic exercise-induced stem cel mobilization can induce ischemic cardiac angiogenesis and upregulate a variety of vascular endothelial growth factors so as to promote myocardial proliferation and repair. However, in-depth exploration is stil needed in the harvesting of stem cel s, transplant rejection, and regulatory mechanisms underlying the directed differentiation of stem cel s into cardiomyocytes.
