CAJ | 학술논문

为了解大黄鱼在低盐胁迫下相关基因的表达变化，采用实时荧光定量 PCR 技术检测了正常盐度25和降低盐度后3个节点(盐度8、盐度3和盐度1.5)鳃、肾、肝、心、脾、肌肉、脑和肠8组织中 gh、igf-1、hsp90和 pparβ基因 mRNA 的表达量变化.研究表明：低盐胁迫可显著影响大黄鱼中这4个基因的表达量. gh 基因在盐度1.5节点除心脏外各组织中表达量均显著高于正常盐度(P<0.05)，其中脾的升幅最大；盐度8和盐度3节点，均是肝中 gh 表达量升高幅度最大，分别为正常盐度的5.21和3.11倍. igf-1基因在盐度1.5节点除肌肉和脑外表达量均显著上调(P<0.05)，鳃中表达量最高，为正常盐度的5.28倍，其次为肾(3.05倍)和肝(2.20倍). hsp90基因在盐度8节点时心和肝中的表达量显著升高(P<0.05)；盐度1.5节点心中表达量显著降低(P<0.05)，而在其他组织中的表达量均显著升高(P<0.05).在盐度8节点 pparβ基因在心、肌肉、肝和脾中的表达量均显著高于其他3个盐度点(P<0.05)；盐度1.5节点鳃、脑、肾和肠中的表达量均显著升高(P<0.05)，分别为正常盐度时的9.04、7.36、3.39和3.19倍.上述结果可为深入研究大黄鱼应对低渗环境的分子调控机制提供基础资料.
위료해대황어재저염협박하상관기인적표체변화，채용실시형광정량 PCR 기술검측료정상염도25화강저염도후3개절점(염도8、염도3화염도1.5)새、신、간、심、비、기육、뇌화장8조직중 gh、igf-1、hsp90화 pparβ기인 mRNA 적표체량변화.연구표명：저염협박가현저영향대황어중저4개기인적표체량. gh 기인재염도1.5절점제심장외각조직중표체량균현저고우정상염도(P<0.05)，기중비적승폭최대；염도8화염도3절점，균시간중 gh 표체량승고폭도최대，분별위정상염도적5.21화3.11배. igf-1기인재염도1.5절점제기육화뇌외표체량균현저상조(P<0.05)，새중표체량최고，위정상염도적5.28배，기차위신(3.05배)화간(2.20배). hsp90기인재염도8절점시심화간중적표체량현저승고(P<0.05)；염도1.5절점심중표체량현저강저(P<0.05)，이재기타조직중적표체량균현저승고(P<0.05).재염도8절점 pparβ기인재심、기육、간화비중적표체량균현저고우기타3개염도점(P<0.05)；염도1.5절점새、뇌、신화장중적표체량균현저승고(P<0.05)，분별위정상염도시적9.04、7.36、3.39화3.19배.상술결과가위심입연구대황어응대저삼배경적분자조공궤제제공기출자료.
In order to investigate the gene expression changes responding to low salt stress, we detected the expression level of gh, igf-1, hsp90 and pparβ gene in different tissues of large yellow crocea (Pseudosciaena crocea) by real-time quantitative PCR. The samples were collected at normal salinity (25) and three low salt nodes (8, 3 and 1.5). The result shows that the expression of these four genes is significantly altered under low salt stress. At salinity 1.5 node, the expression level of gh rises significantly (P<0.05) in all tissues except heart, and the most significant increase of amplitude is observed in spleen. Among all the sampled tissues, liver shows the most notable increase of amplitude of gh expression both at salinity 8 and 3 nodes, and the expression level is 5.21 and 3.11 times the normal salinity, respectively. The expression of igf-1 mRNA goes up significantly (P<0.05) in all tissues at salinity 1.5 node, except muscle and brain. At salinity 1.5 node, the expression level of igf-1 is found in gill, which turns out to be 5.28 fold to normal salinity, and followed by kidney (3.05 fold) and liver (2.20 fold). The expression level of hsp90 rises significantly (P<0.05) in heart and liver at salinity 8 node. At salinity 1.5 node, the expression of hsp90 is declined significantly (P<0.05) in heart, while all the other tissues climbs significantly (P<0.05). At salinity 8 node, the expression level of pparβ in heart, muscle, liver and spleen are all significantly higher than the other three salinities (P<0.05). At salinity 1.5 node, the expression of pparβ in gill, brain, kidney and gut all raised significantly (P<0.05), and are 9.04, 7.36, 3.39 and 3.19 fold to normal salinity. These results are expected to provide fundamental data for further research on the molecular mechanism of response to low salt stress in Pseudosciaena crocea.