CAJ | 학술논문

以北美海蓬子种子为材料,采用 RACE 技术获得北美海蓬子 BADH 基因的 cDNA 全长序列.结果表明： BADH 基因 cDNA 全长为1836 bp,其中开放阅读框(ORF)为1503 bp,编码500个氨基酸,预测蛋白的分子量为54.5 kDa,理论等电点为5.31.推测出 BADH 氨基酸中含有甜菜碱醛脱氢酶家族高度保守的十肽序列(VTLELGGKSP)及与酶功能相关的半胱氨酸残基(Cys).序列比对和系统进化树显示,北美海蓬子与盐节木和盐穗木的亲缘关系最近,同源性达94%.并构建了植物表达载体 pCAMBIA3301-BADH,将其成功导入到农杆菌 EHA105中,为进一步研究该基因的功能奠定了基础.
이북미해봉자충자위재료,채용 RACE 기술획득북미해봉자 BADH 기인적 cDNA 전장서렬.결과표명： BADH 기인 cDNA 전장위1836 bp,기중개방열독광(ORF)위1503 bp,편마500개안기산,예측단백적분자량위54.5 kDa,이론등전점위5.31.추측출 BADH 안기산중함유첨채감철탈경매가족고도보수적십태서렬(VTLELGGKSP)급여매공능상관적반광안산잔기(Cys).서렬비대화계통진화수현시,북미해봉자여염절목화염수목적친연관계최근,동원성체94%.병구건료식물표체재체 pCAMBIA3301-BADH,장기성공도입도농간균 EHA105중,위진일보연구해기인적공능전정료기출.
A full length cDNA sequence of BADH gene was cloned by RACE from Salicornia bigelovii seeds. The results show that the full length cDNA of BADH gene is 1 836 bp, which contains 1 503 bp ORF and encoded 500 amino acid with a putative molecular mass of 54.5 kDa and an isoionic point of 5.31.The deduced amino acid sequence contains the conserved decapeptide sequence (VTLELGGKSP) and cysteine residue in betaine aldehyde dehydrogenase. Sequence comparison and phylogenetic tree analysis reveal that Salicornia bigeloviiwas is close to Halocnemum strobilaceum and Halostachys caspica with 94% amino acids similarity. The plant expression vector pCAMBIA3301-BADH is successfully constructed, and the constructed vector is then transformed into Agrobacterium EHA105. The research is expected to lay a foundation for the further study on function of BADH gene.