CAJ | 학술논문

Thebsd-pg (bundle sheath defective pale green) mutant is a novel maize mutation, controled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue calus of the maize inbred line 501. The char-acterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cels rather than in mesophyl cels. For mapping thebsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using speciifc locus ampliifed fragment sequencing (SLAF-Seq) technology, a total of 5783 polymorphic SLAFs were analysed with 1771 homozygous aleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal aleles of 2:1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the ifne mapping, new simple sequence repeats (SSRs) markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning ofbsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cel types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.