CAJ | 학술논문

制备了一种新型的含2个包覆分子印迹聚合物的丝网印刷碳工作电极的电化学发光夹心免疫传感器阵列，通过该阵列实现了肿瘤标志物癌胚抗原(CEA)和糖类抗原199(CA199)的近同时免疫分析。该新型的电化学发光免疫方法将空间分辨技术与分子印迹技术相结合，为 CEA 和CA199的复合免疫分析提供了一条简单、低成本、快速和灵敏的途径。该方法对临床实验中多种蛋白质的近同时测定也显示了较大的潜力。
제비료일충신형적함2개포복분자인적취합물적사망인쇄탄공작전겁적전화학발광협심면역전감기진렬，통과해진렬실현료종류표지물암배항원(CEA)화당류항원199(CA199)적근동시면역분석。해신형적전화학발광면역방법장공간분변기술여분자인적기술상결합，위 CEA 화CA199적복합면역분석제공료일조간단、저성본、쾌속화령민적도경。해방법대림상실험중다충단백질적근동시측정야현시료교대적잠력。
In this study, a novel electrochemiluminescence (ECL) immunosensor array is engineered consisting of two screen-printed carbon working electrodes coated with molecular imprinting polymer (MIPs). The array is formed to perform multiplexed immunoassay of tumor markers (carcinoembryonic antigen (CEA) and carbohydrate antigen-199 (CA199)). Based on spatial-resolved technique, and coupled with molecularly imprinted technique, this novel ECL strategy provides a simple, low-cost, fast and sensitive approach for multiplexed immunoassay of CEA and CA199. This approach may render promising potential for protein detection in the circumstances of clinical laboratory setting.