CAJ | 학술논문

文中建立了高效液相色谱法测定桑园土壤样品中草甘膦残留量的方法.通过考察柱前衍生、流动相等相关因素的实验条件，结合影响草甘膦检测精确度的单因素实验，确定了草甘膦的最佳柱前衍生高效液相色谱检测方法，并对该方法的准确性和精密度进行了验证.结果表明：草甘膦浓度在5.0~35.0μg/mL范围内，线性关系良好，相关系数（ R2）为0.9992.在0.1~1.0μg/g添加量范围内，桑园土壤中草甘膦的平均回收率为98.5%~103.0%，变异系数为3.54%~4.08%.本实验得到柱前衍生高效液相色谱法检测桑园土壤中草甘膦残留的方法回收率和精密度较好，可满足对桑园土壤草甘膦残留检测的要求.
문중건립료고효액상색보법측정상완토양양품중초감련잔류량적방법.통과고찰주전연생、류동상등상관인소적실험조건，결합영향초감련검측정학도적단인소실험，학정료초감련적최가주전연생고효액상색보검측방법，병대해방법적준학성화정밀도진행료험증.결과표명：초감련농도재5.0~35.0μg/mL범위내，선성관계량호，상관계수（ R2）위0.9992.재0.1~1.0μg/g첨가량범위내，상완토양중초감련적평균회수솔위98.5%~103.0%，변이계수위3.54%~4.08%.본실험득도주전연생고효액상색보법검측상완토양중초감련잔류적방법회수솔화정밀도교호，가만족대상완토양초감련잔류검측적요구.
A method for determination glyphosate residues in soil of Mulberry field was established using high-performance liquid chromatography( HPLC). By investigating the pre-column derivatization,flow equivalence factors related to experimental conditions,and combined with the effects of glyphosate detection accuracy of sin-gle factor,the best HPLC detection method of glyphosate was determined,and the accuracy and precision of the method was verified. The results showed that for the concentration of glyphosate within 5. 0 ~ 35. 0μg/mL, good linear relationship existed,the correlation coefficient( R2 )was 0. 999 2. When addition amount ranged within 0. 1~1. 0μg/g,the average recovery was 98. 5% ~ 103. 0%,the variation coefficient was 3. 54% ~4. 08%,the method had good recovery rate and precision,satisfing the requirement of soil glyphosate residue de-tection of mulberry field.