CAJ | 학술논문

对生物过程中营养物质的消耗进行监测，有利于控制微生物的生长环境，使其始终处于最佳生长条件，从而使目标产物的产量最大化。针对酵母培养液中甘油、甲醇和葡萄糖含量的监测需求，研制了一种基于阿达玛变换（hadamard transform，HT）技术的近红外光谱仪。该光谱仪使用自主改进设计的近红外原位探头进行光谱信号的收集，采用数字微镜（digital micro-mirror devices，DMD）进行阿达玛模板的编码调制，配合自主开发的光谱采集软件和光谱分析处理软件，实现了培养液中营养物含量的实时监测。给出了光谱仪的光路设计、近红外原位探头改进设计、硬件电路和软件模块的设计。通过实验测得该光谱仪的杂散光为0．875％，波动率为±4．28％。利用该光谱仪对一系列标准浓度的甘油水溶液进行光谱采集和数学建模，实验结果表明该仪器测量准确，能够满足生物过程监测的要求。
대생물과정중영양물질적소모진행감측，유리우공제미생물적생장배경，사기시종처우최가생장조건，종이사목표산물적산량최대화。침대효모배양액중감유、갑순화포도당함량적감측수구，연제료일충기우아체마변환（hadamard transform，HT）기술적근홍외광보의。해광보의사용자주개진설계적근홍외원위탐두진행광보신호적수집，채용수자미경（digital micro-mirror devices，DMD）진행아체마모판적편마조제，배합자주개발적광보채집연건화광보분석처리연건，실현료배양액중영양물함량적실시감측。급출료광보의적광로설계、근홍외원위탐두개진설계、경건전로화연건모괴적설계。통과실험측득해광보의적잡산광위0．875％，파동솔위±4．28％。이용해광보의대일계렬표준농도적감유수용액진행광보채집화수학건모，실험결과표명해의기측량준학，능구만족생물과정감측적요구。
Monitoring the consumption of nutrients of biological process helps control the growth environment of the microbes.It ensures the microbes are always in the best growing conditions,so as to maximize the yield of the target product.To monitor the content of glycerol,methanol and glucose in yeast culture medium,a new NIR spectrometer was developed which is based on Hadamard Transform(HT)technique.It uses the Near IR probe,which was designed all by ourselves,to collect the spectral sig-nals,and uses the Digital Micro-mirror Devices(DMD)to complete the coding and modulation of Hadamard template.With self-developed software,aiming at spectral acquisition and spectral processing,the spectrometer has realized the real-time monito-ring.The designs of optical path,Near IR probe,hardware circuit and software modules are given.Through lots of experi-ments,it turns out that the value of stray light is 0.875% and the volatility is ±4.28% in this spectrometer.The spectrometer shows high precision in a series of tests which means it totally meets the requirements of monitoring the biological processes.