CAJ | 학술논문

在高致病性病原微生物实验活动过程中，选择有效的消毒剂对实验室环境去污染非常重要，这也是避免实验室获得性感染发生的有效途径。本研究针对结核分枝杆菌 H37Rv ，应用滤膜过滤悬液定量杀菌实验进行消毒剂验证，其中75．0％乙醇、含有效氯1000 mg／L的次氯酸钠及Safespore消毒剂可高效杀灭结核分枝杆菌，37．5％乙醇作用延长至10 min也可有效杀灭结核分枝杆菌，这为选择针对结核分枝杆菌的生物安全三级实验室表面消毒剂提供了依据。
재고치병성병원미생물실험활동과정중，선택유효적소독제대실험실배경거오염비상중요，저야시피면실험실획득성감염발생적유효도경。본연구침대결핵분지간균 H37Rv ，응용려막과려현액정량살균실험진행소독제험증，기중75．0％을순、함유효록1000 mg／L적차록산납급Safespore소독제가고효살멸결핵분지간균，37．5％을순작용연장지10 min야가유효살멸결핵분지간균，저위선택침대결핵분지간균적생물안전삼급실험실표면소독제제공료의거。
The application of effective disinfectants during experimental activities of high‐pathogenic microorganism is crucial for decontamination of laboratory environment to prevent laboratory‐acquired infections .In this study , the quantitative germicidal disinfectant validation experiments using membrane filtration suspension were conducted against Mycobacterium tuberculosis (M . tuberculosis) H37Rv .Sodium hypochlorite solution (containing 1 000 mg/L active chlorine ) , 75% ethanol/water , and Safespore disinfectant killed M . tuberculosis efficiently .When the concentration of ethanol decreased to 37 .5% ,it was still able to kill M . tuberculosis by prolonging killing time to 10 min . The results suggest that 75%ethanol/water is suitable as conventional disinfectant and sodium hypochlorite solution (containing 1 000 mg/L active chlorine) is suitable under emergency and accident in biosafety level 3 laboratories (BSL‐3) .