CAJ | 학술논문

为提高DNA疫苗的体内转化效率，增强抗原的体内表达，本研究选用慢病毒来源的表达载体pFUW构建新型重组DNA质粒pFUW‐Ag85B‐Rv3425。用重组质粒DNA对C57BL／6小鼠进行肌内免疫后，立即利用电脉冲核酸药物导入仪在DNA免疫部位施以电压的免疫策略，于第3次免疫2周后检测免疫效果。结果显示，新型重组DNA质粒pFUW‐Ag85B‐Rv3425在电击介导下能刺激机体产生更强烈的体液免疫反应，细胞免疫倾向于Th1型，显著增强CD8＋ T细胞和CD27＋ B细胞增殖。研究表明，电击介导重组DNA质粒pFUW‐Ag85B‐Rv3425免疫小鼠能增强免疫效果，有望成为新型的结核分枝杆菌疫苗免疫策略。
위제고DNA역묘적체내전화효솔，증강항원적체내표체，본연구선용만병독래원적표체재체pFUW구건신형중조DNA질립pFUW‐Ag85B‐Rv3425。용중조질립DNA대C57BL／6소서진행기내면역후，립즉이용전맥충핵산약물도입의재DNA면역부위시이전압적면역책략，우제3차면역2주후검측면역효과。결과현시，신형중조DNA질립pFUW‐Ag85B‐Rv3425재전격개도하능자격궤체산생경강렬적체액면역반응，세포면역경향우Th1형，현저증강CD8＋ T세포화CD27＋ B세포증식。연구표명，전격개도중조DNA질립pFUW‐Ag85B‐Rv3425면역소서능증강면역효과，유망성위신형적결핵분지간균역묘면역책략。
A novel DNA vaccine pFUW‐Ag85B‐Rv3425 based on lentiviral vector pFUW was constructed to improve the transfection efficiency in vivo .The DNA injection combined with electroporation in C57BL/6 mice three times with two‐week interval was conducted . After the last immunization , the mice were sacrificed .The spleen lymphocytes were isolated and analyzed by flow cytometry .The expression levels of IgG ,IgG1 ,IgG2a ,and interleukin 2 (IL‐2) in serum were measured by enzyme‐linked immunosorbent assay (ELISA) .The results showed that DNA injection in combination with electroporation could significantly improve humoral immunity ,Th1‐type immune response and CD8+ T cell and CD27+ B cell proliferation . The results indicated that DNA vaccine provided by a combination of injection with electroporation can be taken as a new therapy strategy against tuberculosis .