微生物与感染
微生物與感染
미생물여감염
Journal of Microbes and Infections
2015年
5期
288-293
,共6页
王婷%段歌红%陈茂才%冀恒涛%崔会玲
王婷%段歌紅%陳茂纔%冀恆濤%崔會玲
왕정%단가홍%진무재%기항도%최회령
人巨细胞病毒%病毒DNA%乳汁%尿液%血清阳性率
人巨細胞病毒%病毒DNA%乳汁%尿液%血清暘性率
인거세포병독%병독DNA%유즙%뇨액%혈청양성솔
Human cytomegalovirus%Viral DNA%Breast milk%Urine%Seroprevalence
对华中地区孕妇和住院儿童进行人巨细胞病毒（HCMV ）感染流行病学调查，并分析乳汁、尿液和外周血单个核细胞（PBMC）中HCMV DNA载量对临床的诊断价值。应用电化学发光法检测 HCMV特异性IgM和IgG抗体；采用实时荧光定量聚合酶链反应（PCR ）检测母亲乳汁、尿液和PBMC中 HCMV DNA水平。孕妇和儿童的IgM阳性率分别为1．16％和8．13％（新生儿2．06％，早产儿3．50％），而IgG阳性率分别为96．59％和79．98％（新生儿83．84％，早产儿94．59％）。住院儿童尿液的 HCMV DNA阳性率为57．25％，而PBMC的HCMV DNA阳性率仅为7．97％（ P＜0．001）。母亲乳汁的 HCMV DNA阳性率为44．61％，而PBMC的HCMV DNA阳性率仅为0．77％（ P＜0．001）。结果表明，孕妇 HCMV感染率很高，同时 HCMV是住院儿童感染的重要病原体，且早产儿比足月儿对 HCMV更具易感性。在住院儿童中，尿液标本用于检测HCMV DNA比 PBMC标本具有更高的灵敏度。而哺乳母亲乳汁的 HCMV DNA 阳性率显著高于PBMC ，表明HCMV在乳汁中载量较高，因此哺乳是HCMV的重要母婴传播途径。
對華中地區孕婦和住院兒童進行人巨細胞病毒（HCMV ）感染流行病學調查，併分析乳汁、尿液和外週血單箇覈細胞（PBMC）中HCMV DNA載量對臨床的診斷價值。應用電化學髮光法檢測 HCMV特異性IgM和IgG抗體；採用實時熒光定量聚閤酶鏈反應（PCR ）檢測母親乳汁、尿液和PBMC中 HCMV DNA水平。孕婦和兒童的IgM暘性率分彆為1．16％和8．13％（新生兒2．06％，早產兒3．50％），而IgG暘性率分彆為96．59％和79．98％（新生兒83．84％，早產兒94．59％）。住院兒童尿液的 HCMV DNA暘性率為57．25％，而PBMC的HCMV DNA暘性率僅為7．97％（ P＜0．001）。母親乳汁的 HCMV DNA暘性率為44．61％，而PBMC的HCMV DNA暘性率僅為0．77％（ P＜0．001）。結果錶明，孕婦 HCMV感染率很高，同時 HCMV是住院兒童感染的重要病原體，且早產兒比足月兒對 HCMV更具易感性。在住院兒童中，尿液標本用于檢測HCMV DNA比 PBMC標本具有更高的靈敏度。而哺乳母親乳汁的 HCMV DNA 暘性率顯著高于PBMC ，錶明HCMV在乳汁中載量較高，因此哺乳是HCMV的重要母嬰傳播途徑。
대화중지구잉부화주원인동진행인거세포병독（HCMV ）감염류행병학조사，병분석유즙、뇨액화외주혈단개핵세포（PBMC）중HCMV DNA재량대림상적진단개치。응용전화학발광법검측 HCMV특이성IgM화IgG항체；채용실시형광정량취합매련반응（PCR ）검측모친유즙、뇨액화PBMC중 HCMV DNA수평。잉부화인동적IgM양성솔분별위1．16％화8．13％（신생인2．06％，조산인3．50％），이IgG양성솔분별위96．59％화79．98％（신생인83．84％，조산인94．59％）。주원인동뇨액적 HCMV DNA양성솔위57．25％，이PBMC적HCMV DNA양성솔부위7．97％（ P＜0．001）。모친유즙적 HCMV DNA양성솔위44．61％，이PBMC적HCMV DNA양성솔부위0．77％（ P＜0．001）。결과표명，잉부 HCMV감염솔흔고，동시 HCMV시주원인동감염적중요병원체，차조산인비족월인대 HCMV경구역감성。재주원인동중，뇨액표본용우검측HCMV DNA비 PBMC표본구유경고적령민도。이포유모친유즙적 HCMV DNA 양성솔현저고우PBMC ，표명HCMV재유즙중재량교고，인차포유시HCMV적중요모영전파도경。
This study aims to investigate the epidemiology of human cytomegalovirus ( HCMV ) among pregnant women and hospitalized children in central China area , and to evaluate the value of different samples〔breast milk ,urine and peripheral blood mononuclear cells (PBMCs)〕 for diagnosis of HCMV infection . HCMV‐specific IgM and IgG antibodies were determined by electrochemiluminescence immunoassay to verify the infection status of HCMV .DNA isolated from different samples was subjected for the detection of HCMV‐specific gene(s) by real‐time fluorescence quantitative polymerase chain reaction (PCR) .For pregnant women and hospitalized children ,the positive rate of IgM was 1 .16% and 8 .13%respectively .The rates for newborns and preterm infants were 2 .06% and 3 .50% .The positive rates of IgG for HCMV among pregnant women and hospitalized children were 96 .59% and 79 .98% , respectively , while the rates among newborns and preterm infants were 83 .84% and 94 .59% ,respectively .The positive rates of HCMV DNA detection were 57 .25% and 7 .97% for urine and PBMCs ,respectively (P<0 .001) . 44 .61% of mothers shed HCMV DNA in breast milk ,while 0 .77% were found positive in PBMCs ( P<0 .001) .The results suggest that HCMV infection rate in pregnant women is considerably high and HCMV is one of the pathogens for hospitalized children infection .The preterm infants seem more sensitive to HCMV infection than the term infants ,and the analysis of the presence of viral DNA from urine in hospitalized children is more suitable for the diagnosis of HCMV infection .HCMV DNA has a higher positive rate in breast milk than in PBMCs from lactating mothers and maternal milk is the main source for HCMV transmission from mother to infant .
