CAJ | 학술논문

本文对从内蒙古赤峰巴雅尔草业基地紫花苜蓿根部分离到的菌株 BYE27-2-5进行了致病性测定、形态学和rDNA-ITS 序列分析鉴定及生物学特性测定。结果表明，该菌菌丝生长、产孢和孢子萌发的最适温度分别为25，30和28℃；pH 值5．0～11．0均宜于菌丝生长，产孢最适 pH 8．0，孢子萌发最适 pH 7．0；光暗交替利于菌丝生长，光照利于产孢和孢子的萌发；孢子在相对湿度低于75％不萌发，高于95％萌发较快；葡萄糖蛋白胨培养基利于菌丝生长和孢子萌发，马铃薯蔗糖琼脂培养基利于产孢；碳源中葡萄糖利于菌丝生长和孢子萌发，蔗糖利于产孢；氮源中蛋白胨利于菌丝生长，酵母膏利于产孢和孢子萌发；菌丝致死温度为54℃（10 min），分生孢子的致死温度为48℃（10 min）；根据其形态特征和 rDNA-ITS 序列分析结果，鉴定其为拟枝孢镰刀菌（Fusarium sporotrichioide ）。
본문대종내몽고적봉파아이초업기지자화목숙근부분리도적균주 BYE27-2-5진행료치병성측정、형태학화rDNA-ITS 서렬분석감정급생물학특성측정。결과표명，해균균사생장、산포화포자맹발적최괄온도분별위25，30화28℃；pH 치5．0～11．0균의우균사생장，산포최괄 pH 8．0，포자맹발최괄 pH 7．0；광암교체리우균사생장，광조리우산포화포자적맹발；포자재상대습도저우75％불맹발，고우95％맹발교쾌；포도당단백동배양기리우균사생장화포자맹발，마령서자당경지배양기리우산포；탄원중포도당리우균사생장화포자맹발，자당리우산포；담원중단백동리우균사생장，효모고리우산포화포자맹발；균사치사온도위54℃（10 min），분생포자적치사온도위48℃（10 min）；근거기형태특정화 rDNA-ITS 서렬분석결과，감정기위의지포렴도균（Fusarium sporotrichioide ）。
The strain BYE27-2-5 isolated from alfalfa (Medicago sativa )with root rot in north China was com-prehensively evaluated by analyses of its biological characteristics,morphology,and ITS sequence.The opti-mum temperatures for mycelium growth,sporulation,and spore germination were 25℃,30℃,and 28℃,re-spectively.The strain was able to grow in a pH range of 5.0 -11.0 and the optimal pH for sporulation and spore germination were 8.0 and 7.0,respectively.Alternative light conditions were optimal for mycelial growth whereas complete light conditions were optimal for sporulation and spore germination.Spores germina-ted most rapidly at 95% relative humidity or in water-drop conditions,and failed germinate at relative humidity levels below 75%.Dextrose peptone medium was the best medium for mycelial growth and spore germination and potato dextrose agar was the best medium for sporulation.The best carbon source for mycelial growth and spore germination was glucose and that for sporulation was sucrose.The best nitrogen source for mycelium growth was peptone and that for spore germination and sporulation was yeast extract.The lethal temperature for mycelia was 54℃ (10 min)and that for spores was 48℃ (10 min).Based on these biological characteristics and the results of an ITS sequence analysis,the isolate was identified as Fusarium sporotrichioide .