中南民族大学学报（自然科学版）
中南民族大學學報（自然科學版）
중남민족대학학보（자연과학판）
Journal of South-Central University for Nationalities (Natural Science Edition)
2015年
3期
45-49
,共5页
穗花杉双黄酮%牛血清白蛋白%荧光猝灭
穗花杉雙黃酮%牛血清白蛋白%熒光猝滅
수화삼쌍황동%우혈청백단백%형광졸멸
Amentoflavone%bovine serum albumin%fluorescence quenching
目的：运用荧光猝灭法研究不同温度下穗花杉双黄酮（ AMF）与牛血清白蛋白（ BSA）的相互作用．方法：采用荧光光谱研究AMF与BSA之间的猝灭类型，计算二者之间的结合常数及结合位点数等．结果：穗花衫双黄酮能使BSA发生内源性荧光猝灭，属静态猝灭机制．在298，308，318 K下，AMF与BSA结合常数分别为6．73，6．38，6．17 L· mol-1，结合位点数n近似为1；热力学分析表明AMF与BSA之间结合力为静电力作用；同步荧光光谱表明AMF使BSA构象发生改变，且色氨酸所处微环境的疏水性增强．结论：荧光猝灭法可用于AMF与BSA之间结合反应的研究，方法简便，灵敏度高．
目的：運用熒光猝滅法研究不同溫度下穗花杉雙黃酮（ AMF）與牛血清白蛋白（ BSA）的相互作用．方法：採用熒光光譜研究AMF與BSA之間的猝滅類型，計算二者之間的結閤常數及結閤位點數等．結果：穗花衫雙黃酮能使BSA髮生內源性熒光猝滅，屬靜態猝滅機製．在298，308，318 K下，AMF與BSA結閤常數分彆為6．73，6．38，6．17 L· mol-1，結閤位點數n近似為1；熱力學分析錶明AMF與BSA之間結閤力為靜電力作用；同步熒光光譜錶明AMF使BSA構象髮生改變，且色氨痠所處微環境的疏水性增彊．結論：熒光猝滅法可用于AMF與BSA之間結閤反應的研究，方法簡便，靈敏度高．
목적：운용형광졸멸법연구불동온도하수화삼쌍황동（ AMF）여우혈청백단백（ BSA）적상호작용．방법：채용형광광보연구AMF여BSA지간적졸멸류형，계산이자지간적결합상수급결합위점수등．결과：수화삼쌍황동능사BSA발생내원성형광졸멸，속정태졸멸궤제．재298，308，318 K하，AMF여BSA결합상수분별위6．73，6．38，6．17 L· mol-1，결합위점수n근사위1；열역학분석표명AMF여BSA지간결합력위정전력작용；동보형광광보표명AMF사BSA구상발생개변，차색안산소처미배경적소수성증강．결론：형광졸멸법가용우AMF여BSA지간결합반응적연구，방법간편，령민도고．
Objective:To study the mechanism of interaction between Amentoflavone ( AMF) and Bovine Serum Albumin ( BSA) at different temperatures by fluorescence quenching method .Method: The quenching type , binding constant and binding site was investigated by fluorescence spectrometry . Result: AMF could induce an endogenous fluorescence quenching of BSA under a mechanism of static quenching .The binding constants were detected to be 6.73(298 K), 6.38 (308 K), 6.17(318 K) L· mol-1 and the binding site (n) was about 1, respectively.The thermodynamic parameters suggested that the interaction force between AMF and BSA was electrostatic force .The results of synchronous fluorescence spectra showed AMF can change the conformation of BSA , the hydrophobicity around the tryptophan residues was increased .Conclusion:Fluorescence quenching method could be applied to study the interaction between AMF and BSA .It is a convenient and delicate method .
