CAJ | 학술논문

为研究和筛选甘草提取物中的活性成分，采用超滤质谱法（ UF-MS）研究了人血清白蛋白和5种甘草提取物（甘草苷、甘草酸、甘草素、甘草呋喃酮和甘草利酮）的相互作用。结果表明：这5种甘草提取物均与人血清白蛋白有不同程度的结合，其中甘草酸的结合能力最强，其次是甘草苷、甘草素、甘草呋喃酮和甘草利酮。说明超滤质谱法可准确识别与受体蛋白质结合的活性小分子配体，实现天然产物活性成分的快速筛选。
위연구화사선감초제취물중적활성성분，채용초려질보법（ UF-MS）연구료인혈청백단백화5충감초제취물（감초감、감초산、감초소、감초부남동화감초리동）적상호작용。결과표명：저5충감초제취물균여인혈청백단백유불동정도적결합，기중감초산적결합능력최강，기차시감초감、감초소、감초부남동화감초리동。설명초려질보법가준학식별여수체단백질결합적활성소분자배체，실현천연산물활성성분적쾌속사선。
In order to study and screen the active ingredient in licorice extract , the interaction between human serum albumin and 5 licorice extracts (liquorice glycosides, glycyrrhizic acid, licorice, licorice furfuran ketone and licoricone ) were studied using ultrafiltration mass spectrometry (UF-MS).The results showed that all of the 5 licorice extracts could bind with human serum albumin ( HSA).Among them, glycyrrhizic acid had the highest binding affinity , followed by liquorice glycosides , licorice, licorice furfuran ketone and licoricone .Our work demonstrated that UF-MS could accurately identify the small active molecule ligands of the receptor protein , thus rapidly screen active ingredients from natural products.