CAJ | 학술논문

建立了咖啡豆中绿原酸和咖啡因的超高效液相色谱测定方法. 咖啡豆粉碎后经70 %甲醇水溶液超声提取后,在Waters BEH C18(100 mm×2.1 mm,1.7μm)上以酸性乙腈水为流动相,采用紫外检测器在274 nm测定.在优化试验条件下,咖啡豆中绿原酸和咖啡因提取完全.绿原酸在88.4 mg/L~442 mg/L时峰强度与浓度成良好线性(r2=0.999 3),相对标准偏差为2.81%,平均回收率为98.24%~99.72%.咖啡因在28.8 mg/L~143 mg/L时峰强度与浓度成良好线性(r2=0.999 8),相对标准偏差为1.34%,平均回收率为98.50%~101.32%.方法准确可靠,适用于咖啡豆中绿原酸和咖啡因含量的测定.
건립료가배두중록원산화가배인적초고효액상색보측정방법. 가배두분쇄후경70 %갑순수용액초성제취후,재Waters BEH C18(100 mm×2.1 mm,1.7μm)상이산성을정수위류동상,채용자외검측기재274 nm측정.재우화시험조건하,가배두중록원산화가배인제취완전.록원산재88.4 mg/L~442 mg/L시봉강도여농도성량호선성(r2=0.999 3),상대표준편차위2.81%,평균회수솔위98.24%~99.72%.가배인재28.8 mg/L~143 mg/L시봉강도여농도성량호선성(r2=0.999 8),상대표준편차위1.34%,평균회수솔위98.50%~101.32%.방법준학가고,괄용우가배두중록원산화가배인함량적측정.
A Ultra Performance Liquid Chromatographic method was developed for the determination of chlorogenic acid and caffeine in coffee beans. Coffee bean sample was smashed and ultraphonic extracted with methanol-water (70:30, by volumn). The separation of chlorogenic acid and caffeine were achieved with a Waters BEH C18 (100 mm×2.1 mm, 1.7μm)column by using a mixture of acidic property acetonitrile-water as the mobile phase. The ultraviolet detector was set at 274 nm. The chlorogenic acid and caffeine in coffee bean was extracted completely. There was a good linear relationship between chromatographic peak areas and the concentrations of chlorogenic acid in the range of 88.4 mg/L-442 mg/L with a correlation coefficient of 0.999 3. The RSDs of the method were in the range of 2.81%, and the average recoveries were between 98.24%and 99.72%. There was also a good linear relationship between chromatographic peak areas and the concentrations of caffeine in the range of 28.8 mg/L-143 mg/L with a correlation coefficient of 0.999 8. The RSDs of the method were in the range of 1.34 %, and the average recoveries were between 98.50 % and 101.32 %. The results showed that the method was successfully applied in the analysis of chlorogenic acid and caffeine in coffee bean with its good repeatability and sensitivity.