CAJ | 학술논문

建立大川芎方HPLC指纹图谱方法学.考察色谱条件,选择合适的分析波长,考察方法的精密度、重现性、稳定性等.结果表明:色谱条件:色谱柱:Hypersil ODS2 C18(4.6 mm×250 mm,5μm)反相色谱柱,流动相甲醇-1%醋酸梯度洗脱:0 min→35 min,A:5%→35%;35 min→50 min,A:35%→80%;50 min→60 min,A:80%→80%;60 min→65 min,A:80%→5%;柱温:30℃;流速:1 mL/min;检测波长270 nm.在上述条件下,所建立方法的精密度、重现性、稳定性等良好.所建立的大川芎方HPLC指纹图谱方法学为该方的定性、定量分析提供了新方法.
건립대천궁방HPLC지문도보방법학.고찰색보조건,선택합괄적분석파장,고찰방법적정밀도、중현성、은정성등.결과표명:색보조건:색보주:Hypersil ODS2 C18(4.6 mm×250 mm,5μm)반상색보주,류동상갑순-1%작산제도세탈:0 min→35 min,A:5%→35%;35 min→50 min,A:35%→80%;50 min→60 min,A:80%→80%;60 min→65 min,A:80%→5%;주온:30℃;류속:1 mL/min;검측파장270 nm.재상술조건하,소건립방법적정밀도、중현성、은정성등량호.소건립적대천궁방HPLC지문도보방법학위해방적정성、정량분석제공료신방법.
To develop HPLC chromatographic fingerprint for Dachuanxiong formula. The chromatographic conditions were investigated, the proper analytical wavelength was inspected, and the precision, reproducibility and stability were determined. Chromatographic conditions:A Hypersil ODS2 C18 (4.6 mm × 250 mm,5μm)was applied with the gradient elution solvent system composing of methanol (A) and 1%acetic acid water (B):0 min→35 min,A:5%→35%;35 min→50 min,A:35%→80%;50 min→60 min,A:80%→80%;60 min→65 min,A:80%→5%. The column temperature was 30℃and the flow rate was 1 mL/min.The signal was acquired at 270 nm. Under the conditions we investigated, the precision, reproducibility and stability of the HPLC chromatographic fingerprint method were good. The HPLC chromatographic fingerprint method established in this paper applied a new method for qualitative and quantitative for Dachuanxiong formula.