CAJ | 학술논문

主要研究纯化后苦瓜皂苷的成分组成及对HepG2胰岛素抵抗的缓解效果,为苦瓜皂苷降血糖活性的深入研究提供参考.本试验利用AB-8大孔吸附树脂纯化苦瓜皂苷粗提物,以人参皂苷Rg1和Rb1为参照物,通过薄层层析(TLC)技术初步分析纯化后样品的组成,然后利用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)验证分析的结果,最后采用高浓度胰岛素诱导HepG2细胞建立胰岛素抵抗模型,考察不同剂量的苦瓜皂苷对葡萄糖消耗量的影响.结果表明,经AB-8大孔吸附树脂纯化后苦瓜皂苷含量由35.8%提高到了69.1%,薄层层析显色分析提取物中至少含有5种皂苷类物质,HPLC-ELSD得出纯化后苦瓜皂苷样品中除含有人参皂苷Rg1和人参皂苷Rb1外,还有含有其他6种组分.与模型组细胞相比,纯化后的苦瓜皂苷在一定程度上可以改善产生HepG2/IR细胞的葡萄糖消耗量,其中300μg/mL的苦瓜皂苷效果最好.
주요연구순화후고과조감적성분조성급대HepG2이도소저항적완해효과,위고과조감강혈당활성적심입연구제공삼고.본시험이용AB-8대공흡부수지순화고과조감조제물,이인삼조감Rg1화Rb1위삼조물,통과박층층석(TLC)기술초보분석순화후양품적조성,연후이용고효액상색보-증발광산사검측기(HPLC-ELSD)험증분석적결과,최후채용고농도이도소유도HepG2세포건립이도소저항모형,고찰불동제량적고과조감대포도당소모량적영향.결과표명,경AB-8대공흡부수지순화후고과조감함량유35.8%제고도료69.1%,박층층석현색분석제취물중지소함유5충조감류물질,HPLC-ELSD득출순화후고과조감양품중제함유인삼조감Rg1화인삼조감Rb1외,환유함유기타6충조분.여모형조세포상비,순화후적고과조감재일정정도상가이개선산생HepG2/IR세포적포도당소모량,기중300μg/mL적고과조감효과최호.
The components and insulin resistance ability of purified Momordica charantia saponins were investigated to further study the hyperglycemic effect of it.AB-8 macroporous adsorption resin was used to purify crude saponins.The components of purified saponins were analyzed by thinlayer chromatography (TLC) and high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD).The insulin resistant HepG2 cell model was established by high concentration insulin.Different concentration Momordica charantia saponins were added to measure glucose consumption by glucose oxidase method (GOD).These results showed that the content of saponins increased from 35.8%to 69.1%.There were less than 5 kinds of saponins based on TLC technique.By HPLC-ELSD,there were other 6 kinds of saponins,besides Ginsenoside Rg1 and Rb1.Compared with HepG2/IR group,treatment with 300μg/mL Momordica charantia saponins could enhance glucose consumption of HepG2 cells/IR.