CAJ | 학술논문

目的检测角蛋白(K)-4在口腔黏膜下纤维性变(OSF or OSMF)早、中、晚期组织中的表达,初步探讨K4在OSF发生、发展过程中的作用机制,为OSF的治疗提供新的靶点,为其疗效判断提供新的有效监测物.方法收集10例正常颊黏膜组织;30例未经任何治疗的OSF患者颊黏膜组织,其中早期、中期、晚期各10例.运用免疫组化和Western blot蛋白印迹方法检测上述组织中K4的表达.结果免疫组化结果显示,K4的表达主要定位在细胞质,阳性细胞可见于上皮层全层,呈棕黄色颗粒.在正常颊黏膜中,K4呈强阳性表达,高于OSF早、中、晚期病变组,差异均有统计学意义(P＜0.05);K4在OSF早、中、晚期表达呈递减趋势,晚期显著低于早期,差异有统计学意义(P＜0.05).Western blot结果显示,在正常颊黏膜中K4的表达量高于OSF早、中、晚病变组,差异均具有统计学意义(P＜0.05);在OSF早、中、晚期颊黏膜组织中,K4的表达呈递减趋势,但三组之间差异无统计学意义(P＞0.05).结论 K4在OSF早、中、晚期组织中的表达降低,可能参与了OSF发生发展过程.
목적 검측각단백(K)-4재구강점막하섬유성변(OSF or OSMF)조、중、만기조직중적표체,초보탐토K4재OSF발생、발전과정중적작용궤제,위OSF적치료제공신적파점,위기료효판단제공신적유효감측물.방법 수집10례정상협점막조직;30례미경임하치료적OSF환자협점막조직,기중조기、중기、만기각10례.운용면역조화화Western blot단백인적방법검측상술조직중K4적표체.결과 면역조화결과현시,K4적표체주요정위재세포질,양성세포가견우상피층전층,정종황색과립.재정상협점막중,K4정강양성표체,고우OSF조、중、만기병변조,차이균유통계학의의(P＜0.05);K4재OSF조、중、만기표체정체감추세,만기현저저우조기,차이유통계학의의(P＜0.05).Western blot결과현시,재정상협점막중K4적표체량고우OSF조、중、만병변조,차이균구유통계학의의(P＜0.05);재OSF조、중、만기협점막조직중,K4적표체정체감추세,단삼조지간차이무통계학의의(P＞0.05).결론 K4재OSF조、중、만기조직중적표체강저,가능삼여료OSF발생발전과정.
Objective To investigate the expressions of cytokeratin/keratin 4 in the buccal tissue of oral submucous fibrosis at the early,middle and advanced stages,explore which role keratin4 (K4) plays in the process and development of oral submucous fibrosis (OSF),and provide evidence for K4 being a promising biomarker to evaluate the development and prognosis of OSF.Methods Ten cases of normal tissues,and 10 cases of OSF tissues with typical early,middle and advanced stages,were selected,respectively.Detect the expression of K4 in the tissue mentioned above through immunohistochemistry and Westem blot.The data was analyzed by statistical means.Results The results of immunohistochemistry showed that K4 was mainly located in cytoplasm,and positive cells with brownish yellow granules were seen in whole epithelial layer of the normal mucosa.The expression of K4 was lower at all stages of OSF than that at the normal tissue with statistical significance (P ＜0.05).With the aggravation of OSF,the expression of K4 was decreased,difference between early and advanced stage was found to be statistically significant.The results of Western blot also showed that the expression of K4 was lower than that of early,middle and advanced stages of OSF (P ＜ 0.05).With the aggravation of OSF,the expression of K4 was decreased,but the differences between them had no significance (P ＞ 0.05).Conclusions The expression of K4 in OSF tissue of early,middle and advanced stages were decreased compared to normal tissue,respectively.It suggests that K4 might play and important role in the initiation and development of OSF.