CAJ | 학술논문

以新鲜猪皮为原料,分别采用中性蛋白酶、菠萝蛋白酶、胃蛋白酶、木瓜蛋白酶、胰蛋白酶5种不同蛋白酶水解胶原蛋白,以水解度(DH)和超氧阴离子自由基(O2-·)清除率为指标,根据单因素和正交试验优化5种蛋白酶水解猪皮胶原蛋白的最适条件;比较分析5种蛋白酶对猪皮胶原蛋白的水解效果.结果表明:5种蛋白酶对猪皮胶原蛋白的水解产物均具有抗氧化性,其水解产物对O2-·清除率依次为:胰蛋白酶水解产物>中性蛋白酶水解产物>胃蛋白酶水解产物>菠萝蛋白酶水解产物>木瓜蛋白酶水解产物.
이신선저피위원료,분별채용중성단백매、파라단백매、위단백매、목과단백매、이단백매5충불동단백매수해효원단백,이수해도(DH)화초양음리자자유기(O2-·)청제솔위지표,근거단인소화정교시험우화5충단백매수해저피효원단백적최괄조건;비교분석5충단백매대저피효원단백적수해효과.결과표명:5충단백매대저피효원단백적수해산물균구유항양화성,기수해산물대O2-·청제솔의차위:이단백매수해산물>중성단백매수해산물>위단백매수해산물>파라단백매수해산물>목과단백매수해산물.
With fresh pigskin as material,five different proteases including neutral protease,bromelain,pepsin,papain,trypsin were used to hydrolyze collagen.Taking DH and scavenging rate of O2-·as index,the condition of the five proteases hydrolyzing pigskin collagen was optimized according to single factor and orthogonal test,and comparatively study the five proteases effect on pigskin collagen.It turned out that:hydrolysates of the five proteases were all anti-oxidative,and the scavenging rates of O2-·were as follows:trypsin>neutral protease>pepsin>bromelain>papain.