食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
17期
67-69
,共3页
马鞭草%总三萜酸%正交试验
馬鞭草%總三萜痠%正交試驗
마편초%총삼첩산%정교시험
Verbena officinalis L.%total triterpenoids%orthogonal test
优选马鞭草中总三萜酸的提取工艺.采用紫外分光光度法测定总三萜酸含量;以总三萜酸得率为指标,采用单因素试验考察乙醇浓度、料液比、提取时间、提取次数的影响,在此基础上设计正交试验优选马鞭草中总三萜酸的提取工艺.最佳提取工艺为80%乙醇35倍量超声提取3次,每次30 min.在此条件下,总三萜酸得率21.91 mg/g.该工艺稳定可行,为马鞭草中三萜酸的开发利用提供参考.
優選馬鞭草中總三萜痠的提取工藝.採用紫外分光光度法測定總三萜痠含量;以總三萜痠得率為指標,採用單因素試驗攷察乙醇濃度、料液比、提取時間、提取次數的影響,在此基礎上設計正交試驗優選馬鞭草中總三萜痠的提取工藝.最佳提取工藝為80%乙醇35倍量超聲提取3次,每次30 min.在此條件下,總三萜痠得率21.91 mg/g.該工藝穩定可行,為馬鞭草中三萜痠的開髮利用提供參攷.
우선마편초중총삼첩산적제취공예.채용자외분광광도법측정총삼첩산함량;이총삼첩산득솔위지표,채용단인소시험고찰을순농도、료액비、제취시간、제취차수적영향,재차기출상설계정교시험우선마편초중총삼첩산적제취공예.최가제취공예위80%을순35배량초성제취3차,매차30 min.재차조건하,총삼첩산득솔21.91 mg/g.해공예은정가행,위마편초중삼첩산적개발이용제공삼고.
To optimize the extraction technology of total triterpenoids from Verbena officinalis L..The content of total triterpenoids was determined by UV with oleanic as reference; Effects of concentration of extraction solvent,solid-liquid ratio,extraction time and extraction times by taking yield of total triterpenoids as the index,basis on this,extraction technology was optimized by orthogonal test.The optimum extraction technology were as follow:extracted 3 times with 35 times the amount of 80%ethanol,30 min each time.Under these conditions,yield of total triterpenoids from Verbena officinalis L.was 21.91 mg/g.This extraction process was stable and feasible,which provide the reference for the development and utilization of triterpenoids from Verbena officinalis.