CAJ | 학술논문

为了筛选具有产业化应用前景的富油微藻,以实验室保藏的4株淡水和海洋微藻为研究对象,在室外自然条件下利用光径为3 cm和5 cm板式光生物反应器通气分批培养,通过测定微藻培养物的生物量和总脂含量等指标, 从中筛选生长速度快、生物量和总脂含量高的微藻. 结果表明:4株微藻的生物量产率和总脂含量分别在0.099 g/(L·d)~0.201 g/(L·d)和20.4%dw~40.1%dw(%Dry weight)之间,分别是眼点拟微绿球藻(Nannochloropsisoculata) [0.201 g/(L·d),40.1%dw]、荒漠栅列藻(Scenedesmusdeserticola)[0.099 g/(L·d),30.8%dw]、双形栅藻(Scenedesmusdimorphus) [0.142 g/(L·d),20.4%dw]、若夫小球藻(Chlorallazofingensis)[0.155 g/(L·d),29.3%dw],其中最具产业化潜力的微藻为眼点拟微绿球藻(Nannochloropsisoculata),其总脂产量和单位体积总脂产率分别为1.409 g/L和108.353 mg/(L·d),其中二十碳五烯酸(EPA)含量占干重含量的7.2%,是较优的EPA生产原料.
위료사선구유산업화응용전경적부유미조,이실험실보장적4주담수화해양미조위연구대상,재실외자연조건하이용광경위3 cm화5 cm판식광생물반응기통기분비배양,통과측정미조배양물적생물량화총지함량등지표, 종중사선생장속도쾌、 생물량화총지함량고적미조. 결과표명:4주미조적생물양산솔화총지함량분별재0.099 g/(L·d)~0.201 g/(L·d)화20.4%dw~40.1%dw(%Dry weight)지간,분별시안점의미록구조(Nannochloropsisoculata) [0.201 g/(L·d),40.1%dw]、황막책렬조(Scenedesmusdeserticola)[0.099 g/(L·d),30.8%dw]、쌍형책조(Scenedesmusdimorphus) [0.142 g/(L·d),20.4%dw]、약부소구조(Chlorallazofingensis)[0.155 g/(L·d),29.3%dw],기중최구산업화잠력적미조위안점의미록구조(Nannochloropsisoculata),기총지산량화단위체적총지산솔분별위1.409 g/L화108.353 mg/(L·d),기중이십탄오희산(EPA)함량점간중함량적7.2%,시교우적EPA생산원료.
Four strains of freshwater and marine microalgae collected in laboratory were screened for their potential of oil production for industrial application. All microalgae were cultured in batch , with flat plate photobioreactors (light path, 3 cm and 5 cm). These microalgae were evaluated by measuring biomuss, total lipid content, total lipid yield, total lipid volumetric productivity. The results showed that the biomuss yield and total lipid contents of four microalgae were in the range of 0.099 g/(L·d)-0.201 g/(L·d) and 20.4%dw-40.1%dw, respectively,including Nannochloropsisoculata[0.201 g/(L·d),40.1%dw],Scenedesmusdeserticola[0.099g/(L·d), 30.8%dw],Scenedesmusdimorphus [0.142 g/(L·d),20.4%dw],Chlorallazofingensis [0.155 g/(L·d),29.3%dw]. It was concluded that the most potential for industrial application was Nannochloropsisoculata, which lipid yield and lipid volumetric productivity was 1.409 g/L and 108.353 mg/(L·d), respectively. The content of EPA was 7.2%dw,was the more excellent EPA production of raw materials.