食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
18期
44-49
,共6页
刘英丽%李福芳%张慧娟%王静%邸斯婕
劉英麗%李福芳%張慧娟%王靜%邸斯婕
류영려%리복방%장혜연%왕정%저사첩
甜菜%色素%微波辅助提取%响应面优化法%稳定性
甜菜%色素%微波輔助提取%響應麵優化法%穩定性
첨채%색소%미파보조제취%향응면우화법%은정성
beet%pigment%microwave assisted extraction%response surface methodology (RSM)%stability
通过单因素试验对甜菜红色素提取条件(料液比、温度、提取时间、提取液pH、提取溶剂等)进行初步探究,并通过微波辅助对色素提取进行响应面优化设计,结果表明影响提取最显著的因素是微波时间,其次是微波功率,最后是料液比.最适提取条件:料液比1:5.76(g/mL),微波功率398 W,微波时间112.8 s,此条件甜菜红色素最大吸光度值为0.711 4.对提取的甜菜红色素进行超滤处理并对其粗提液进行稳定性研究,结果表明光照、pH对其稳定性影响显著,添加不同稳定剂对甜菜红色素稳定性有一定改善,苯甲酸钠(0.04%)和VC(0.04%)复配使用对于提高其稳定性作用较为明显.
通過單因素試驗對甜菜紅色素提取條件(料液比、溫度、提取時間、提取液pH、提取溶劑等)進行初步探究,併通過微波輔助對色素提取進行響應麵優化設計,結果錶明影響提取最顯著的因素是微波時間,其次是微波功率,最後是料液比.最適提取條件:料液比1:5.76(g/mL),微波功率398 W,微波時間112.8 s,此條件甜菜紅色素最大吸光度值為0.711 4.對提取的甜菜紅色素進行超濾處理併對其粗提液進行穩定性研究,結果錶明光照、pH對其穩定性影響顯著,添加不同穩定劑對甜菜紅色素穩定性有一定改善,苯甲痠鈉(0.04%)和VC(0.04%)複配使用對于提高其穩定性作用較為明顯.
통과단인소시험대첨채홍색소제취조건(료액비、온도、제취시간、제취액pH、제취용제등)진행초보탐구,병통과미파보조대색소제취진행향응면우화설계,결과표명영향제취최현저적인소시미파시간,기차시미파공솔,최후시료액비.최괄제취조건:료액비1:5.76(g/mL),미파공솔398 W,미파시간112.8 s,차조건첨채홍색소최대흡광도치위0.711 4.대제취적첨채홍색소진행초려처리병대기조제액진행은정성연구,결과표명광조、pH대기은정성영향현저,첨가불동은정제대첨채홍색소은정성유일정개선,분갑산납(0.04%)화VC(0.04%)복배사용대우제고기은정성작용교위명현.
The appropriate extraction conditions of betalain from beets by single factor experiment (solvent ratio, temperature, extraction time, pH, extraction solvent) were determined. On the basis of that conclusion, using response surface methodology (RSM) for optimizing extraction of betalain investigated the effects of solvent/material ratio, microwave output power and micro-wave time. The results showed that among the three factors the microwave time most significantly affected on the absorbance , followed by the microwave output power and the solvent/material ratio. The optimal conditions were as follows:solvent/material ratio 1:5.76(g/mL), microwave output power 398 W and microwave time 112.8 s, the highest absorbance was 0.711 4. Through ultrafiltration and the stability was studied, research found that the sunshine and pH had significant impact on the stability. Different stabilizers have a certain improvement on the stability of betalain , and using of sodium benzoate (0.04%) and VC (0.04%) together can improve its stability significantly.