心血管康复医学杂志
心血管康複醫學雜誌
심혈관강복의학잡지
Chinese Journal of Cardiovascular Rehabilitation Medicine
2015年
5期
540-543
,共4页
缺血后处理%再灌注损伤%细胞凋亡
缺血後處理%再灌註損傷%細胞凋亡
결혈후처리%재관주손상%세포조망
Ischemic postconditioning%Reperfusion injury%Apoptosis
目的：研究硝酸酯药物后处理对大鼠缺血再灌注损伤的心肌保护作用。方法：Wistar 大鼠32只随机分为假手术组、缺血再灌注组（I／R组）、后处理组（IPostC组）及硝酸酯后处理组（PI‐PostC组），每组8只。分别测定各组缺血和梗死心肌面积、血浆心肌肌钙蛋白I （cTnI）水平和细胞凋亡指数，并进行比较。结果：与假手术组比较， I／R组， IPostC组和PI‐PostC组的cTnI水平均显著升高[（6.39±2.35）μg／L比（70.33±6.94）μg／L比（41.19±4.50）μg／L、（37.47±4.20）μg／L ， P＜0.01]，且 IPostC组和PI‐PostC组的显著低于 I／R组， P均＜0.01。与I／R组比较， IPostC 组和 PI‐PostC 组缺血面积[（53.31±3.87）％比（46.46±2.13）％比（42.08±4.84）％]，梗死心肌面积明显减小[（52.19±3.44）％比（41.02±2.93）％、（38.13±2.05）％]，细胞凋亡指数明显降低[（26.92±1.91）％比（20.54±3.05）％、（19.49±2.41）％]， P均＜0.01。与IPostC组比较， PI‐PostC组缺血心肌面积显著减小（P＜0.05），但梗死心肌面积、cTnI水平和细胞凋亡指数无明显改变（ P＞0.05）。结论：硝酸盐药物后处理与缺血后处理对心肌缺血再灌注损伤具有同样的保护作用，并且能更有效地减小心肌缺血面积。
目的：研究硝痠酯藥物後處理對大鼠缺血再灌註損傷的心肌保護作用。方法：Wistar 大鼠32隻隨機分為假手術組、缺血再灌註組（I／R組）、後處理組（IPostC組）及硝痠酯後處理組（PI‐PostC組），每組8隻。分彆測定各組缺血和梗死心肌麵積、血漿心肌肌鈣蛋白I （cTnI）水平和細胞凋亡指數，併進行比較。結果：與假手術組比較， I／R組， IPostC組和PI‐PostC組的cTnI水平均顯著升高[（6.39±2.35）μg／L比（70.33±6.94）μg／L比（41.19±4.50）μg／L、（37.47±4.20）μg／L ， P＜0.01]，且 IPostC組和PI‐PostC組的顯著低于 I／R組， P均＜0.01。與I／R組比較， IPostC 組和 PI‐PostC 組缺血麵積[（53.31±3.87）％比（46.46±2.13）％比（42.08±4.84）％]，梗死心肌麵積明顯減小[（52.19±3.44）％比（41.02±2.93）％、（38.13±2.05）％]，細胞凋亡指數明顯降低[（26.92±1.91）％比（20.54±3.05）％、（19.49±2.41）％]， P均＜0.01。與IPostC組比較， PI‐PostC組缺血心肌麵積顯著減小（P＜0.05），但梗死心肌麵積、cTnI水平和細胞凋亡指數無明顯改變（ P＞0.05）。結論：硝痠鹽藥物後處理與缺血後處理對心肌缺血再灌註損傷具有同樣的保護作用，併且能更有效地減小心肌缺血麵積。
목적：연구초산지약물후처리대대서결혈재관주손상적심기보호작용。방법：Wistar 대서32지수궤분위가수술조、결혈재관주조（I／R조）、후처리조（IPostC조）급초산지후처리조（PI‐PostC조），매조8지。분별측정각조결혈화경사심기면적、혈장심기기개단백I （cTnI）수평화세포조망지수，병진행비교。결과：여가수술조비교， I／R조， IPostC조화PI‐PostC조적cTnI수평균현저승고[（6.39±2.35）μg／L비（70.33±6.94）μg／L비（41.19±4.50）μg／L、（37.47±4.20）μg／L ， P＜0.01]，차 IPostC조화PI‐PostC조적현저저우 I／R조， P균＜0.01。여I／R조비교， IPostC 조화 PI‐PostC 조결혈면적[（53.31±3.87）％비（46.46±2.13）％비（42.08±4.84）％]，경사심기면적명현감소[（52.19±3.44）％비（41.02±2.93）％、（38.13±2.05）％]，세포조망지수명현강저[（26.92±1.91）％비（20.54±3.05）％、（19.49±2.41）％]， P균＜0.01。여IPostC조비교， PI‐PostC조결혈심기면적현저감소（P＜0.05），단경사심기면적、cTnI수평화세포조망지수무명현개변（ P＞0.05）。결론：초산염약물후처리여결혈후처리대심기결혈재관주손상구유동양적보호작용，병차능경유효지감소심기결혈면적。
Objective:To study protective effect of nitrates pharmacological postconditioning (PI‐PostC) on ischemia‐reperfusion (I/R) injured myocardium in rats .Methods :A total of 32 Wistar rats were randomly and equally divided into sham operation group ,I/R group ,ischemia postconditioning group (IPostC group) and PI‐PostC group .Myo‐cardial ischemic area ,infract size ,plasma level of cardiac troponin I (cTnI) and cell apoptotic index were measured and compared among all groups .Results:Compare with sham operation group ,there was significant rise in plasma cTnI level [ (6.39 ± 2.35)μg/L vs .(70.33 ± 6.94)μg/L vs .(41.19 ± 4.50)μg/L ,(37.47 ± 4.20)μg/L ,P<0.01 all] in I/R group ,IPostC group and PI‐PostC group ,and those of IPostC group and PI‐PostC group were significant‐ly lower than that of I/R group , P<0.01 both .Compared with I/R group ,there were significant reductions in my‐ocardial ischemic area [ (53.31 ± 3.87)% vs .(46.46 ± 2.13)% vs .(42.08 ± 4.84)% ] ,infarct size [ (52.19 ± 3.44)% vs .(41.02 ± 2.93 )% , (38.13 ± 2.05 )% ] and cell apoptotic index [ (26.92 ± 1.91 )% vs . (20.54 ± 3.05)% ,(19.49 ± 2.41)% ] in IPostC group and PI‐PostC group ,P<0.01 all .Compared with IPostC group ,ische‐mic area significantly reduced (P<0.05) in IP‐PostC group ,but there were no significant changes in infarct size , cTnI level and cell apoptotic index between IPostC group and IP‐PostC group , P>0.05. Conclusion:Nitrates phar‐macological postconditioning possesses the same protective effect on myocardial I/R injury as ischemia postcondition‐ing ,and it can reduce myocardial ischemic area more effectively .
