CAJ | 학술논문

以树莓干果为研究材料,总黄酮提取率为评价指标.先分析了超声时间、浸提温度和乙醇浓度各单因素对树莓总黄酮提取率的影响,再通过响应面法和正交法比较最佳提取工艺和产率,结果显示,响应面法最佳工艺为超声时间42.30 min,浸提温度80℃,乙醇浓度51.83 %(约52 %),理论预测值3.83 %,实际验证值3.88 %,并且超声时间和乙醇浓度存在交互作用;SPSS正交法的最佳工艺为超声时间40 min,浸提温度70℃,乙醇浓度50 %,实际验证值是3.78 %.虽然2种方法得出相似的最佳工艺,但响应面法能提供更多更精确的信息,最终产率也略高,值得推广.
이수매간과위연구재료,총황동제취솔위평개지표.선분석료초성시간、침제온도화을순농도각단인소대수매총황동제취솔적영향,재통과향응면법화정교법비교최가제취공예화산솔,결과현시,향응면법최가공예위초성시간42.30 min,침제온도80℃,을순농도51.83 %(약52 %),이론예측치3.83 %,실제험증치3.88 %,병차초성시간화을순농도존재교호작용;SPSS정교법적최가공예위초성시간40 min,침제온도70℃,을순농도50 %,실제험증치시3.78 %.수연2충방법득출상사적최가공예,단향응면법능제공경다경정학적신식,최종산솔야략고,치득추엄.
Dry raspberry fruits were used as experimental materials with the total flavonoids extraction rate as a measure indicator. Three extraction parameters including ultrasound time , extraction temperature and ethanol concentration were optimized by response surface methodology (RSM) and orthogonal design based on single factor investigations for achieving the maximum extraction rate of the total flavonoids. The results showed that the theoretical value of RSM was 3.83 %under the optimum condition of ultrasound time 42.30 min , extraction temperature 80℃and ethanol concentration 51.83% (about 52%), and the actual extracting rate of the total flavonoids was 3.88 %.There was the interaction between ultrasound time and ethanol concentration. Moreover , the actual extraction rate of orthogonal design under the optimum condition of ultrasound time 40 min , extraction temperature 70 ℃ and ethanol concentration 50 %was 3.78 %. Although the optimum extraction conditions from two methods were the similarity, the RSM provided the more actual data information and got the higher extraction rate, which was worth of being generalized.