CAJ | 학술논문

利用DPPH、Fenton反应、SNAP、黄嘌呤氧化酶氧化黄嘌呤反应产生活性有机氮自由基、羟自由基(·OH)、一氧化氮(NO)自由基、超氧阴离子(·O2-)自由基,以电子顺磁共振法(ESR)研究了螺旋藻体外清除上述四种自由基的作用.该方法操作简单易行,结果直观.结果表明螺旋藻能有效清除上述4种自由基,而且随浓度的增加抗氧化能力也增加,浓度在40 mg/mL时对超氧阴离子和有机氮自由基的清除率达到了90%以上.
이용DPPH、Fenton반응、SNAP、황표령양화매양화황표령반응산생활성유궤담자유기、간자유기(·OH)、일양화담(NO)자유기、초양음리자(·O2-)자유기,이전자순자공진법(ESR)연구료라선조체외청제상술사충자유기적작용.해방법조작간단역행,결과직관.결과표명라선조능유효청제상술4충자유기,이차수농도적증가항양화능력야증가,농도재40 mg/mL시대초양음리자화유궤담자유기적청제솔체도료90%이상.
Organic nitrogen radicals, hydroxyl radicals (·OH), nitric oxide (NO) radicals, superoxide anion radicals (·O2-) were produced by DPPH, Fenton reaction, SNAP, oxidation reaction of xanthine and xanthine oxidase. The antioxidant activities of spirulina in different concentrations were determined by the four radicals scavenging activity using electron paramagnetic resonance (ESR)Technology. The method was simple and easy to observe the results. Experimental results showed that spirulina have strong free radical scavenging capacity. The free radical scavenging capacity was increased by increasing concentrations. when the concentration in 40 mg/mL , the scavenging rates of Superoxide anion and organic nitrogen radicals reached more than 90%.