CAJ | 학술논문

目的观察垂体腺瘤组织环指蛋白1(RING1)的表达及其与垂体腺瘤KNOSP分级的关系.方法收集南方医科大学南方医院神经外科自2011年1月至2013年12月手术切除并经病理证实的人脑垂体腺瘤组织标本60例,其中KNOSP分级Ⅰ～Ⅱ级17例,Ⅲ～Ⅳ级43例.免疫组化染色检测垂体腺瘤组织RING1、细胞核增殖相关抗原(Ki-67)的表达.结果与Ⅰ～Ⅱ级组比较,Ⅲ～Ⅳ级组垂体腺瘤标本中RING1、Ki-67阳性细胞数和阳性率均较高,差异均有统计学意义(P＜0.05).不同性别、年龄、功能分型垂体腺瘤患者标本RING1的表达强度比较差异无统计学意义(P＜0.05);与KNOSP分级Ⅰ～Ⅱ级垂体腺瘤(47.1％)比较,Ⅲ～Ⅳ级垂体腺瘤标本中RING1高表达率(76.7％)较高,差异有统计学意义(P＜0.05).结论垂体腺瘤RING1的表达与KNOSP分级有关,可能是垂体腺瘤防治的新靶点.
목적 관찰수체선류조직배지단백1(RING1)적표체급기여수체선류KNOSP분급적관계.방법 수집남방의과대학남방의원신경외과자2011년1월지2013년12월수술절제병경병리증실적인뇌수체선류조직표본60례,기중KNOSP분급Ⅰ～Ⅱ급17례,Ⅲ～Ⅳ급43례.면역조화염색검측수체선류조직RING1、세포핵증식상관항원(Ki-67)적표체.결과 여Ⅰ～Ⅱ급조비교,Ⅲ～Ⅳ급조수체선류표본중RING1、Ki-67양성세포수화양성솔균교고,차이균유통계학의의(P＜0.05).불동성별、년령、공능분형수체선류환자표본RING1적표체강도비교차이무통계학의의(P＜0.05);여KNOSP분급Ⅰ～Ⅱ급수체선류(47.1％)비교,Ⅲ～Ⅳ급수체선류표본중RING1고표체솔(76.7％)교고,차이유통계학의의(P＜0.05).결론 수체선류RING1적표체여KNOSP분급유관,가능시수체선류방치적신파점.
Objective To study ring finger protein 1 (RING1) gene expression in pituitary adenomas and its association with KNOSP grading.Methods Sixty human hypophysis cerebri adenoma tissue specimens,accepted surgery and conformed by pathology in our hospital from January 2011 and December 2013,were chosen in our study;KNOSP grade Ⅰ-Ⅱ was noted 17 specimens and KNOSP grade Ⅲ-Ⅳ was noted in 43 specimens.Immunohistochemical staining was employed to detect the expressions of RING1 and Ki-67 in pituitary adenomas.Results Specimens from KNOSP grade Ⅲ-Ⅳ had significantly larger numbers of RING1 and Ki-67 positive cells than those from KNOSP grade Ⅰ-Ⅱ (P＜0.05).Pituitary adenoma specimens from different gender,age and functional classifications showed no significant difference in the RING1 expression (P＞0.05);RING1 expression percentage in specimens from KNOSP grade Ⅲ-Ⅳ (76.7％) was significantly higher than that in those from grade Ⅰ-Ⅱ (47.1％,P＜0.05).Conclusion RING1 expression is positively correlated with KNOSP grading,and deeper study about RING1 could help to find new treatment target of pituitary adenoma.