中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
Chinese Journal of Behavioral Medicine and Brain Science
2015年
9期
784-786
,共3页
环氧合酶2抑制剂%小神经胶质细胞%海马%神经元
環氧閤酶2抑製劑%小神經膠質細胞%海馬%神經元
배양합매2억제제%소신경효질세포%해마%신경원
Cyclooxygenase 2 inhibitors%Microglia%Hippocampus%Neurons
目的 观察环氧合酶2(Cyclooxygenase 2,COX-2)特异性抑制剂NS398对脂多糖(lipopolysaccharide,LPS)激活小胶质细胞介导的海马神经元损伤是否具有保护作用.方法 分离培养新生SD鼠脑小胶质细胞,采用含有l μg/ml的LPS培养基培养,观察小胶质细胞形态学变化.检测小胶质细胞培养液中IL-1β、TNF-α水平变化.采用不同培养基培养原代新生SD鼠海马神经元:空白组(C组),采用正常小胶质细胞培养基、LPS刺激小胶质细胞后的条件培养基组(L组)、条件培养基加NS398(N组).MTT法测定各组神经元存活率.测定各组海马神经元内乳酸盐与丙酮酸盐比值以评价神经元内氧利用状况.结果 LPS刺激后,小胶质细胞突起明显缩短变粗、浓染,培养基中IL-1β、TNF-α较对照组明显增高(P＜0.01).LPS刺激小胶质细胞后条件培养基作用海马神经元存活率为64.37％,而加入NS398后神经元存活率为80.25％,差异有统计学意义(P＜0.01).L组乳酸盐/丙酮酸盐比值(27.34±8.53)明显高于C组(14.95±4.72),N组(20.32±6.05),组间比较差异有统计学意义(P＜0.01).结论 COX-2特异性抑制剂NS398对小胶质细胞激活后介导的海马神经元的损害具有保护作用.
目的 觀察環氧閤酶2(Cyclooxygenase 2,COX-2)特異性抑製劑NS398對脂多糖(lipopolysaccharide,LPS)激活小膠質細胞介導的海馬神經元損傷是否具有保護作用.方法 分離培養新生SD鼠腦小膠質細胞,採用含有l μg/ml的LPS培養基培養,觀察小膠質細胞形態學變化.檢測小膠質細胞培養液中IL-1β、TNF-α水平變化.採用不同培養基培養原代新生SD鼠海馬神經元:空白組(C組),採用正常小膠質細胞培養基、LPS刺激小膠質細胞後的條件培養基組(L組)、條件培養基加NS398(N組).MTT法測定各組神經元存活率.測定各組海馬神經元內乳痠鹽與丙酮痠鹽比值以評價神經元內氧利用狀況.結果 LPS刺激後,小膠質細胞突起明顯縮短變粗、濃染,培養基中IL-1β、TNF-α較對照組明顯增高(P＜0.01).LPS刺激小膠質細胞後條件培養基作用海馬神經元存活率為64.37％,而加入NS398後神經元存活率為80.25％,差異有統計學意義(P＜0.01).L組乳痠鹽/丙酮痠鹽比值(27.34±8.53)明顯高于C組(14.95±4.72),N組(20.32±6.05),組間比較差異有統計學意義(P＜0.01).結論 COX-2特異性抑製劑NS398對小膠質細胞激活後介導的海馬神經元的損害具有保護作用.
목적 관찰배양합매2(Cyclooxygenase 2,COX-2)특이성억제제NS398대지다당(lipopolysaccharide,LPS)격활소효질세포개도적해마신경원손상시부구유보호작용.방법 분리배양신생SD서뇌소효질세포,채용함유l μg/ml적LPS배양기배양,관찰소효질세포형태학변화.검측소효질세포배양액중IL-1β、TNF-α수평변화.채용불동배양기배양원대신생SD서해마신경원:공백조(C조),채용정상소효질세포배양기、LPS자격소효질세포후적조건배양기조(L조)、조건배양기가NS398(N조).MTT법측정각조신경원존활솔.측정각조해마신경원내유산염여병동산염비치이평개신경원내양이용상황.결과 LPS자격후,소효질세포돌기명현축단변조、농염,배양기중IL-1β、TNF-α교대조조명현증고(P＜0.01).LPS자격소효질세포후조건배양기작용해마신경원존활솔위64.37％,이가입NS398후신경원존활솔위80.25％,차이유통계학의의(P＜0.01).L조유산염/병동산염비치(27.34±8.53)명현고우C조(14.95±4.72),N조(20.32±6.05),조간비교차이유통계학의의(P＜0.01).결론 COX-2특이성억제제NS398대소효질세포격활후개도적해마신경원적손해구유보호작용.
Objective To investigate the protective effect of NS398,a specific cyclooxygenase-2 inhibitor,on microglial activation-mediated neuronal damage.Methods The microglia of neonatal SD rat were isolated and cultured in the medium containing 1 μg/ml of LPS.The morphological changes of microglial were observed.The IL-1β and TNF-α levels were detected by Western blot in LPS group (group L) and control group (group C).The hippocampal neurons of neonatal SD rats were cultured in the control medium (group C),LPS-activated microglial conditioned medium (group L) and LPS-activated microglial conditioned medium with NS398 (group N),respectively.The survival rate of neurons were detected by MTT.The respiration of hippocampus neurons was determined by detecting the ratio of lactic acid/pyruvic acid.Results LPS-induced microglial activation was characterized by the morphological change and increased secretion of IL-1β and TNF-α.The survival rate of neurons cultured by microglia activated conditioned medium was 64.37％,while the group N was 80.25％ (P＜0.01).In group L,the ratio of lactic acid/pyruvic acid (27.34±8.53) was significantly higher than that of group N (20.32±6.05,P＜0.01) and group C (14.95±4.72,P＜0.01).Conclusion NS398,a specific cyclooxygenase-2 inhibitor,has a protective effect on rat hippocampus neuron damaged by activated microglia.
