中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
Chinese Journal of Neuromedicine
2015年
9期
918-922
,共5页
张元峰%张建党%王昆鹏%孙瑞迅%刘素杰%张东波%赵光纪
張元峰%張建黨%王昆鵬%孫瑞迅%劉素傑%張東波%趙光紀
장원봉%장건당%왕곤붕%손서신%류소걸%장동파%조광기
蛛网膜下腔出血%低氧诱导因子-1α%脑损伤%细胞凋亡
蛛網膜下腔齣血%低氧誘導因子-1α%腦損傷%細胞凋亡
주망막하강출혈%저양유도인자-1α%뇌손상%세포조망
Subarachnoid hemorrhage%Hypoxia-inducible factor-1α%Brain injury%Apoptosis
目的 探讨大脑皮层低氧诱导因子-1α(HIF-1 α)与蛛网膜下腔出血(SAH)后早期脑损伤的相关性.方法 选取55只成年健康雄性Sprague-Dawley大鼠,按随机数字表法分为假手术组、SAH 6 h组、SAH 12 h组、SAH 24 h组及SAH72 h组(n=11),其中后4组通过改良的血管内穿刺法建立SAH模型并在相应时间点采取颈椎脱臼法处死.应用TUNEL法检测各组大鼠神经元细胞凋亡情况,应用免疫荧光染色检测HIF-1α的表达水平,应用免疫荧光双标染色检测表达HIF-1α的细胞类型;应用Pearson相关法分析TUNEL阳性率与HIF-1α表达阳性率之间的相关性.结果 与假手术组相比,各SAH组TUNEL阳性率和HIF-1α表达阳性率均显著升高,差异均有统计学意义(P<0.05).免疫荧光双标染色显示神经元特异性核蛋白(NeuN)染色阳性细胞与多数HIF-1α表达阳性细胞重合,胶质纤维酸性蛋白(GFAP)染色阳性细胞与少数HIF-1α表达阳性细胞重合.同时,部分TUNEL染色阳性细胞与HIF-1α表达阳性细胞重合,Pearson相关分析显示HIF-1α表达阳性率与TUNEL阳性率呈正相关关系(r=0.737,P=0.001).结论 在SAH后早期脑损伤的病理生理过程中,HIF-1α高表达加剧了神经元的凋亡.
目的 探討大腦皮層低氧誘導因子-1α(HIF-1 α)與蛛網膜下腔齣血(SAH)後早期腦損傷的相關性.方法 選取55隻成年健康雄性Sprague-Dawley大鼠,按隨機數字錶法分為假手術組、SAH 6 h組、SAH 12 h組、SAH 24 h組及SAH72 h組(n=11),其中後4組通過改良的血管內穿刺法建立SAH模型併在相應時間點採取頸椎脫臼法處死.應用TUNEL法檢測各組大鼠神經元細胞凋亡情況,應用免疫熒光染色檢測HIF-1α的錶達水平,應用免疫熒光雙標染色檢測錶達HIF-1α的細胞類型;應用Pearson相關法分析TUNEL暘性率與HIF-1α錶達暘性率之間的相關性.結果 與假手術組相比,各SAH組TUNEL暘性率和HIF-1α錶達暘性率均顯著升高,差異均有統計學意義(P<0.05).免疫熒光雙標染色顯示神經元特異性覈蛋白(NeuN)染色暘性細胞與多數HIF-1α錶達暘性細胞重閤,膠質纖維痠性蛋白(GFAP)染色暘性細胞與少數HIF-1α錶達暘性細胞重閤.同時,部分TUNEL染色暘性細胞與HIF-1α錶達暘性細胞重閤,Pearson相關分析顯示HIF-1α錶達暘性率與TUNEL暘性率呈正相關關繫(r=0.737,P=0.001).結論 在SAH後早期腦損傷的病理生理過程中,HIF-1α高錶達加劇瞭神經元的凋亡.
목적 탐토대뇌피층저양유도인자-1α(HIF-1 α)여주망막하강출혈(SAH)후조기뇌손상적상관성.방법 선취55지성년건강웅성Sprague-Dawley대서,안수궤수자표법분위가수술조、SAH 6 h조、SAH 12 h조、SAH 24 h조급SAH72 h조(n=11),기중후4조통과개량적혈관내천자법건립SAH모형병재상응시간점채취경추탈구법처사.응용TUNEL법검측각조대서신경원세포조망정황,응용면역형광염색검측HIF-1α적표체수평,응용면역형광쌍표염색검측표체HIF-1α적세포류형;응용Pearson상관법분석TUNEL양성솔여HIF-1α표체양성솔지간적상관성.결과 여가수술조상비,각SAH조TUNEL양성솔화HIF-1α표체양성솔균현저승고,차이균유통계학의의(P<0.05).면역형광쌍표염색현시신경원특이성핵단백(NeuN)염색양성세포여다수HIF-1α표체양성세포중합,효질섬유산성단백(GFAP)염색양성세포여소수HIF-1α표체양성세포중합.동시,부분TUNEL염색양성세포여HIF-1α표체양성세포중합,Pearson상관분석현시HIF-1α표체양성솔여TUNEL양성솔정정상관관계(r=0.737,P=0.001).결론 재SAH후조기뇌손상적병리생리과정중,HIF-1α고표체가극료신경원적조망.
Objective To explore the relationship between hypoxia-inducible factor-1α (HIF-1α) expression and apoptosis in early brain injury models after subarachnoid hemorrhage (SAH).Methods Fifty-five adult male Sprague-Dawley rats were randomly assigned to five groups:sham-operated group,SAH 6 h,SAH 12 h,SAH 24 h and SAH 72 h groups (n=1 1).SAH in the later four groups was induced by modified monofilament puncture method.The rats were killed by cervical dislocation.HIF-1α expression was assessed by immunofluorescence staining.TUNEL was adopted to detect brain apoptotic cells.Immunofluorescence double staining was used to identify cell types with positive HIF-1α expression.Pearson correlation analysis was employed to analyze the relationship between HIF-1 expression percentage and TUNEL positive rate.Results As compared with those in the sham-operated group,the HIF-1 expression percentage and TUNEL positive rate in the four SAH groups was significantly higher (P<0.05).Immunofluorescence double staining showed that neuron-specific nuclear protein staining cells were coincided with most HIF-1 positive cells,while only a few HIF-1α positive cells were coincided with glial fibrillary acidic protein staining cells.A significant positive correlation was noted between HIF-1 α expression percentage and TUNEL positive rate following SAH (r=0.737,P=0.001).Conclusion HIF-1α high expression after SAH early promotes neuronal cell apoptosis,indicating HIF-1 a might participate in the pathological progression of early brain injury after SAH.