CAJ | 학술논문

目的观察肾性高血压大鼠大脑皮层β淀粉样蛋白(β-amyloid protein,Aβ)和学习记忆变化以及灯盏生脉胶囊治疗效果.方法 SD雄性大鼠分为正常组、假手术组、高血压灯盏生脉胶囊治疗组和高血压生理盐水治疗组,每组5只.双肾双夹法构建肾性高血压大鼠模型.灯盏生脉胶囊溶解于0.9％生理盐水中,按照体质量20 mg·kg-1每天灌胃给药,连续4周.高血压生理盐水组给予同剂量生理盐水灌胃.免疫荧光染色观察脑组织Aβ表达,Western blot分析Aβ、NF-κB p65、IL-1β、TNF-α蛋白水平变化.Morris水迷宫测试大鼠学习记忆功能.结果高血压大鼠大脑皮层Aβ蛋白沉积增多,伴随学习记忆功能减退,与假手术组比较,差异有统计学意义(P＜0.01).与高血压生理盐水治疗组比较,口服灯盏生脉胶囊治疗4周血压水平降低[(157.45± 11.58) mmHgvs(197.76± 10.12) mmHg];下调脑Aβ、NF-κB p65、IL-1β、TNF-α蛋白水平,Morris水迷宫测试显示逃避潜伏期缩短[(24.64±4.57)s vs (37.17±3.87)s],经过平台次数[(5.39±0.12)次vs(3.05±0.28)次]和平台象限停留时间增加[(27.34±3.67)s vs (16.83±5.76)s],均P＜0.01.结论灯盏生脉胶囊治疗可能通过发挥抗炎作用减少高血压大鼠大脑皮层Aβ沉积和改善其学习记忆功能.
목적 관찰신성고혈압대서대뇌피층β정분양단백(β-amyloid protein,Aβ)화학습기억변화이급등잔생맥효낭치료효과.방법 SD웅성대서분위정상조、가수술조、고혈압등잔생맥효낭치료조화고혈압생리염수치료조,매조5지.쌍신쌍협법구건신성고혈압대서모형.등잔생맥효낭용해우0.9％생리염수중,안조체질량20 mg·kg-1매천관위급약,련속4주.고혈압생리염수조급여동제량생리염수관위.면역형광염색관찰뇌조직Aβ표체,Western blot분석Aβ、NF-κB p65、IL-1β、TNF-α단백수평변화.Morris수미궁측시대서학습기억공능.결과 고혈압대서대뇌피층Aβ단백침적증다,반수학습기억공능감퇴,여가수술조비교,차이유통계학의의(P＜0.01).여고혈압생리염수치료조비교,구복등잔생맥효낭치료4주혈압수평강저[(157.45± 11.58) mmHgvs(197.76± 10.12) mmHg];하조뇌Aβ、NF-κB p65、IL-1β、TNF-α단백수평,Morris수미궁측시현시도피잠복기축단[(24.64±4.57)s vs (37.17±3.87)s],경과평태차수[(5.39±0.12)차vs(3.05±0.28)차]화평태상한정류시간증가[(27.34±3.67)s vs (16.83±5.76)s],균P＜0.01.결론 등잔생맥효낭치료가능통과발휘항염작용감소고혈압대서대뇌피층Aβ침적화개선기학습기억공능.
Objective To observe the effects of Dengzhan Shengmai capsule on β-amyloid protein (Aβ) deposition in the brain and learning and memory function in renovascular hypertensive rats (RHRSP).Methods Male Sprague-Dawley rats were randomly divided into four groups (5 rats per group):normal group,shamoperated group,hypertension with Dengzhan Shengmai capsule treatment group and hypertension with normal saline (NS) treatment group.Renovascular hypertensive models were created by clipping two-kidney.Dengzhan Shengmai capsules were dissolved in sterile 0.9％ NS and were administered (20 mg · kg-1 per day) by daily gavage for 4 weeks.In the NS group,hypertensive rats were given saline in the same volume.Immunofluorescent labeling and western blot were used to detect the expression of Aβ,NF-κB,IL-1β,TNF-α in the brain,respectively.Learning and memory function were detected by Morris water maze.Results RHRSP significantly increased Aβ deposition in the cerebral cortex and impaired memory function in rats.Dengzhan Shengmai capsule treatment significantly lowered the blood pressure compared with NS treatment((157.45±11.58) mmHgvs (197.76±10.12) mmHg).In addition,the levels of Aβ,NF-κB p65,IL-1β,TNF-α protein were significantly reduced,by Dengzhan Shengmai caspule treatment.The escape latency was shortened((24.64±4.57) s vs (37.17±3.87)s),while the frequency of passing through the platform quadrant(5.39±0.12 vs 3.05±0.28) and the dwell time((27.34±3.67) s vs (16.83±5.76)s) (all P＜0.01) in the platform quadrant were significantly increased by Dengzhan Shengmai capsule treatment.Conclusions Dengzhan Shengmai capsule may reduce Aβ deposition in brain and improve learning and memory function by anti-inflammatory effects in RHRSP.