CAJ | 학술논문

目的通过对全基因组启动子甲基化程度与视觉记忆强相关的基因进行生物信息学分析,寻找与记忆功能相关的基因所在信号通路.方法对9名正常人外周血单核细胞DNA样本进行甲基化DNA免疫共沉淀芯片分析.将基因的启动子区域甲基化水平与即刻及延迟视觉记忆功能进行相关性分析,筛选出与两者都强相关的基因并进一步进行通路和物理交互作用分析.结果与即刻及延迟视觉记忆都强相关的基因有69个,这些基因的通路及物理交互作用分析共涉及42条通路,其中22条通路的Q值小于0.05,包括了与能量代谢、突触功能、酪氨酸激酶活性、白细胞功能相关的通路.结论本研究首次利用甲基化组学数据与数量性状进行相关性及通路分析,揭示了可能与记忆相关并受甲基化调控的信号通路.
목적 통과대전기인조계동자갑기화정도여시각기억강상관적기인진행생물신식학분석,심조여기억공능상관적기인소재신호통로.방법 대9명정상인외주혈단핵세포DNA양본진행갑기화DNA면역공침정심편분석.장기인적계동자구역갑기화수평여즉각급연지시각기억공능진행상관성분석,사선출여량자도강상관적기인병진일보진행통로화물리교호작용분석.결과 여즉각급연지시각기억도강상관적기인유69개,저사기인적통로급물리교호작용분석공섭급42조통로,기중22조통로적Q치소우0.05,포괄료여능량대사、돌촉공능、락안산격매활성、백세포공능상관적통로.결론 본연구수차이용갑기화조학수거여수량성상진행상관성급통로분석,게시료가능여기억상관병수갑기화조공적신호통로.
Objective To explore the biological processes and pathways associated with memory function which may be regulated by gene promoter methylation.Methods The genome-wide promoter methylation statuses in 9 healthy individuals were analyzed with a Multiplex HG18 CpG Promoter chip.Genes with promoter methylation statuses strongly correlated with both immediate and delayed visual memory function were proceeded for pathway and physical interactions analysis.Resnlts Sixty nine genes have been correlated with both immediate and delayed visual memory functions.Twenty two pathways, with a Q-value of ＜ 0.05, were identified by the pathway and physical interactions analysis, which included energy metabolism, axon guidance, tyrosine kinase activity, anterograde synaptic vesicle transport, and leukocyte migration and differentiation.Conclusion Pathways related with memory function may be regulated by DNA methylation.