中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
Chinese Journal of Medical Genetics
2015年
5期
707-710
,共4页
梁文华%周兆山%吉中强%王燕青%薛卫林%张小燕
樑文華%週兆山%吉中彊%王燕青%薛衛林%張小燕
량문화%주조산%길중강%왕연청%설위림%장소연
哮喘%TNF-α基因%IL-13基因%单核苷酸多态性%关联研究
哮喘%TNF-α基因%IL-13基因%單覈苷痠多態性%關聯研究
효천%TNF-α기인%IL-13기인%단핵감산다태성%관련연구
Asthma%Tumor necrosis factor-α gene%Interleukin-13 gene%Single nucleotide polymorphisms%Association analyses
目的 探讨肿瘤坏死因子-α (tumor necrosis factor-α,TNF-α)基因和白细胞介素13(interleukin-13,IL-13)基因多态性与青岛地区汉族成人哮喘的相关性.方法 应用SNaPshot方法对400例哮喘患者和200名正常对照的TNF-α基因的rs1799724、rs1800630、rs1799964和rs769178以及IL-13基因的rs2158177和rs1295687共6个多态位点的多态性进行检测.结果 相对于AA-GA基因型,rs2158177位点GG基因型频率在对照组为5％,哮喘组为2.8％,差异有统计学意义(OR=0.31,95％CI:0.12-0.82,P=0.021).其余5个位点的基因型频率在哮喘组与对照组间差异无统计学意义(P＞0.05).结论 IL-13基因rs2158177多态性与青岛地区汉族成人哮喘具有相关性.未发现TNF-α基因的多态性与青岛地区汉族成人哮喘存在相关性.
目的 探討腫瘤壞死因子-α (tumor necrosis factor-α,TNF-α)基因和白細胞介素13(interleukin-13,IL-13)基因多態性與青島地區漢族成人哮喘的相關性.方法 應用SNaPshot方法對400例哮喘患者和200名正常對照的TNF-α基因的rs1799724、rs1800630、rs1799964和rs769178以及IL-13基因的rs2158177和rs1295687共6箇多態位點的多態性進行檢測.結果 相對于AA-GA基因型,rs2158177位點GG基因型頻率在對照組為5％,哮喘組為2.8％,差異有統計學意義(OR=0.31,95％CI:0.12-0.82,P=0.021).其餘5箇位點的基因型頻率在哮喘組與對照組間差異無統計學意義(P＞0.05).結論 IL-13基因rs2158177多態性與青島地區漢族成人哮喘具有相關性.未髮現TNF-α基因的多態性與青島地區漢族成人哮喘存在相關性.
목적 탐토종류배사인자-α (tumor necrosis factor-α,TNF-α)기인화백세포개소13(interleukin-13,IL-13)기인다태성여청도지구한족성인효천적상관성.방법 응용SNaPshot방법대400례효천환자화200명정상대조적TNF-α기인적rs1799724、rs1800630、rs1799964화rs769178이급IL-13기인적rs2158177화rs1295687공6개다태위점적다태성진행검측.결과 상대우AA-GA기인형,rs2158177위점GG기인형빈솔재대조조위5％,효천조위2.8％,차이유통계학의의(OR=0.31,95％CI:0.12-0.82,P=0.021).기여5개위점적기인형빈솔재효천조여대조조간차이무통계학의의(P＞0.05).결론 IL-13기인rs2158177다태성여청도지구한족성인효천구유상관성.미발현TNF-α기인적다태성여청도지구한족성인효천존재상관성.
Objective To assess the association of polymorphisms of TNF-α gene (rs1799724, rs1800630, rs1799964 and rs769178) and IL-13 gene (rs2158177 and rs1295687) with susceptibility to asthma among ethnic Chinese in Qingdao region.Methods For 400 asthma patients and 200 healthy subjects, above polymorphisms were detected with a SNaPshot method.Results For rs2158177, thefrequency of genotype of GG in the asthma group was significantly lower than the control group (2.8％ vs.5％, OR=0.31, 95％CI: 0.12-0.82,P=0.021).No significant difference was detected in the genotypic frequencies for the remaining 5 polymorphisms between the two groups (All P＞0.05).Conclusion The study has indicated that rs2158177 polymorphism of the IL-13 gene is associated with asthma in ethnic Han Chinese from Qingdao.No association has been found between polymorphisms of TNF-α gene with susceptibility to asthma.
