中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
Chinese Journal of General Surgery
2015年
10期
789-792
,共4页
姚庆娟%卫江鹏%何向辉%刘刚
姚慶娟%衛江鵬%何嚮輝%劉剛
요경연%위강붕%하향휘%류강
结直肠肿瘤%微RNAs%基因表达
結直腸腫瘤%微RNAs%基因錶達
결직장종류%미RNAs%기인표체
Colorectal neoplasms%MicroRNAs%Gene expression
目的 探讨miRNA-21和磷酸酶-张力蛋白基因(phosphatase and tensin homolog,PTEN)在结直肠癌浸润转移中的作用及临床意义.方法 以65例结直肠癌手术标本为研究对象,采用实时定量PCR方法检测肿瘤和切缘组织miRNA-21的mRNA表达水平,采用实时定量PCR和免疫组织化学方法检测PTEN基因的mRNA与蛋白表达水平,并结合临床病理资料进行t检验,方差分析及Pearson相关性分析.结果 肿瘤组织中miRNA-21的mRNA表达水平明显高于切缘组织(=3.50,P＜0.05),PTEN的mRNA及蛋白表达水平均明显低于切缘组织(t=7.35,t=12.23;P ＜0.05);miRNA-21的mRNA、PTEN的mRNA及PTEN蛋白在不同的结直肠癌的浸润深度(F=18.36,F=17.26,F=12.83;P ＜0.05)、Dukes分期(F=31.25,F=24.43,F =57.12;P ＜0.05)以及是否有淋巴结转移(=5.85,t=2.18,t =4.05;P ＜0.05)中的表达水平差异有统计学意义,与分化程度和肿瘤部位无关[F =7.39,t =4.62;F =7.78,t=1.29;F =5.14,t=1.37;均P＞0.05)];结直肠癌中miRNA-21的表达与PTEN的基因和蛋白表达呈负相关(r=-0.994,r=-0.927;P ＜0.05).结论 miRNA-21与PTEN的表达改变与结直肠癌的浸润转移有关.
目的 探討miRNA-21和燐痠酶-張力蛋白基因(phosphatase and tensin homolog,PTEN)在結直腸癌浸潤轉移中的作用及臨床意義.方法 以65例結直腸癌手術標本為研究對象,採用實時定量PCR方法檢測腫瘤和切緣組織miRNA-21的mRNA錶達水平,採用實時定量PCR和免疫組織化學方法檢測PTEN基因的mRNA與蛋白錶達水平,併結閤臨床病理資料進行t檢驗,方差分析及Pearson相關性分析.結果 腫瘤組織中miRNA-21的mRNA錶達水平明顯高于切緣組織(=3.50,P＜0.05),PTEN的mRNA及蛋白錶達水平均明顯低于切緣組織(t=7.35,t=12.23;P ＜0.05);miRNA-21的mRNA、PTEN的mRNA及PTEN蛋白在不同的結直腸癌的浸潤深度(F=18.36,F=17.26,F=12.83;P ＜0.05)、Dukes分期(F=31.25,F=24.43,F =57.12;P ＜0.05)以及是否有淋巴結轉移(=5.85,t=2.18,t =4.05;P ＜0.05)中的錶達水平差異有統計學意義,與分化程度和腫瘤部位無關[F =7.39,t =4.62;F =7.78,t=1.29;F =5.14,t=1.37;均P＞0.05)];結直腸癌中miRNA-21的錶達與PTEN的基因和蛋白錶達呈負相關(r=-0.994,r=-0.927;P ＜0.05).結論 miRNA-21與PTEN的錶達改變與結直腸癌的浸潤轉移有關.
목적 탐토miRNA-21화린산매-장력단백기인(phosphatase and tensin homolog,PTEN)재결직장암침윤전이중적작용급림상의의.방법 이65례결직장암수술표본위연구대상,채용실시정량PCR방법검측종류화절연조직miRNA-21적mRNA표체수평,채용실시정량PCR화면역조직화학방법검측PTEN기인적mRNA여단백표체수평,병결합림상병리자료진행t검험,방차분석급Pearson상관성분석.결과 종류조직중miRNA-21적mRNA표체수평명현고우절연조직(=3.50,P＜0.05),PTEN적mRNA급단백표체수평균명현저우절연조직(t=7.35,t=12.23;P ＜0.05);miRNA-21적mRNA、PTEN적mRNA급PTEN단백재불동적결직장암적침윤심도(F=18.36,F=17.26,F=12.83;P ＜0.05)、Dukes분기(F=31.25,F=24.43,F =57.12;P ＜0.05)이급시부유림파결전이(=5.85,t=2.18,t =4.05;P ＜0.05)중적표체수평차이유통계학의의,여분화정도화종류부위무관[F =7.39,t =4.62;F =7.78,t=1.29;F =5.14,t=1.37;균P＞0.05)];결직장암중miRNA-21적표체여PTEN적기인화단백표체정부상관(r=-0.994,r=-0.927;P ＜0.05).결론 miRNA-21여PTEN적표체개변여결직장암적침윤전이유관.
Objective To explore the correlations between expression of miRNA-21 and PTEN and the invasion, metastasis of colorectal carcinoma.Methods The mRNA level of miRNA-21 and mRNA and protein levels of PTEN were assayed by real-time quantitative PCR and immunohistochemical methods respectively in 65 colorectal carcinoma specimen.Results The expression of miRNA-21 mRNA in cancer tissues was significantly higher than that in paracancerous tissues (t =3.50, P ＜ 0.05).Both mRNA and protein expressions of PTEN in tumor tissue were significantly lower than that in paracancerous tissues (t =7.35,t =12.23;P ＜ 0.05).Expression of miRNA-21 mRNA, PTEN mRNA and protein were obviously related with depth of invasion(F =18.36 ,F =17.26 ,F =12.83;P ＜ 0.05), Dukes stage (F =31.25, F =24.43, F =57.12;P ＜ 0.05) and lymph node metastasis (t =5.85, t =2.18, t =4.05;P ＜ 0.05), while it was not related to tumor differenciation and tumor location (F =7.39, t =4.62;F =7.78, t =1.29;F =5.14,t =1.37;P ＞ 0.05).Positive relation between miRNA-21 and PTEN in colorectal carcinoma was identified (r =-0.994, r =-0.927;P ＜ 0.05).Conclusion High expression of miRNA-21 and low expression of PTEN are both closely associated with invasion and metastasis of colorectal carcinoma.
