实用医学杂志
實用醫學雜誌
실용의학잡지
The Journal of Practical Medicine
2015年
20期
3291-3294
,共4页
蒋校文%张翼%范晓升%邓轩
蔣校文%張翼%範曉升%鄧軒
장교문%장익%범효승%산헌
张应力%骨髓间充质干细胞%硫化氢
張應力%骨髓間充質榦細胞%硫化氫
장응력%골수간충질간세포%류화경
Tensile stress%Bone marrow-derived mesenchymal cells%Hydrogen sulfide
目的:研究张应力下大鼠骨髓间充质细胞(BMMCs)成骨向分化过程中硫化氢(H2S)信号系统的作用。方法:利用四点弯曲加力系统对大鼠BMMCs施加4000μ strain的单一周期张应力60 min ,同时利用工具药物改变单元系统的H 2S信号系统表达( A组细胞单元中加入炔丙基甘氨酸;B组细胞单元中加入磷酸缓冲液;C组细胞单元中加入GYY3147),4 h后检测H2S和胱硫醚-γ-裂解酶的表达量,同时检测碱性磷酸酶、骨钙素表、总骨Ⅰ型前胶原氨基酸延长链和成骨细胞转录因子-2表达水平。结果:上调H2S的表达可促进张应力下BMMCs成骨标记物的上调表达,抑制内源性H2S后,成骨标记物的含量明显下降。结论:内源性H2S信号系统在张应力下BMMCs成骨向分化过程中起着重要作用,上调H2S表达可促进成骨。
目的:研究張應力下大鼠骨髓間充質細胞(BMMCs)成骨嚮分化過程中硫化氫(H2S)信號繫統的作用。方法:利用四點彎麯加力繫統對大鼠BMMCs施加4000μ strain的單一週期張應力60 min ,同時利用工具藥物改變單元繫統的H 2S信號繫統錶達( A組細胞單元中加入炔丙基甘氨痠;B組細胞單元中加入燐痠緩遲液;C組細胞單元中加入GYY3147),4 h後檢測H2S和胱硫醚-γ-裂解酶的錶達量,同時檢測堿性燐痠酶、骨鈣素錶、總骨Ⅰ型前膠原氨基痠延長鏈和成骨細胞轉錄因子-2錶達水平。結果:上調H2S的錶達可促進張應力下BMMCs成骨標記物的上調錶達,抑製內源性H2S後,成骨標記物的含量明顯下降。結論:內源性H2S信號繫統在張應力下BMMCs成骨嚮分化過程中起著重要作用,上調H2S錶達可促進成骨。
목적:연구장응력하대서골수간충질세포(BMMCs)성골향분화과정중류화경(H2S)신호계통적작용。방법:이용사점만곡가력계통대대서BMMCs시가4000μ strain적단일주기장응력60 min ,동시이용공구약물개변단원계통적H 2S신호계통표체( A조세포단원중가입결병기감안산;B조세포단원중가입린산완충액;C조세포단원중가입GYY3147),4 h후검측H2S화광류미-γ-렬해매적표체량,동시검측감성린산매、골개소표、총골Ⅰ형전효원안기산연장련화성골세포전록인자-2표체수평。결과:상조H2S적표체가촉진장응력하BMMCs성골표기물적상조표체,억제내원성H2S후,성골표기물적함량명현하강。결론:내원성H2S신호계통재장응력하BMMCs성골향분화과정중기착중요작용,상조H2S표체가촉진성골。
Objective To evaluate the impact of hydrogen sulfide signal system during the process of rat bone marrow-derived mesenchymal cells osteogenesis under tensile stress. Methods After the H2S signal system of cell rooms regulated by tool drugs (Group A with propargylglycine;Goup B with PBS;Group C with GYY3147), 4 000 μ strain tensile stress were applied on rat BMMCs by four-point bending apparatus for 60 minutes. Four hours later, the H2S signal system and cystathionine-γ-lyase were detected. Meanwhile, the change of the alkaline phosphate, osteocalcin, procollagen typeⅠN-terminal propeptide, and runt-related transcription factor 2 mRNA level were also examined to evaluate the osteogenic ability. Results With the increase of H2S expression, the osteogenic capacity gradually increased, while the osteogenic capacity was compromised after the endogenous cystathionine-γ-lyase was inhibited. Conclusion The H2S signal system plays an important role during BMMCs osteogensis under tensile stress. The up-regulation expression of H2S may promote osteogenesis during distraction osteogenesis.