CAJ | 학술논문

目的：探讨不同Kellgren-Lawrence X线分级的骨性关节炎患者膝关节软骨及软骨下骨中MicroRNA-214(miR-214)的表达，为进一步研究miR-214在骨性关节炎发生和发展中的作用提供依据。方法收集截肢患者及骨性关节炎全膝关节置换患者膝关节胫骨平台40例，根据K-L评分标准将其分为Ⅰ级组(10例)、Ⅱ级组(10例)、Ⅲ级组(10例)、Ⅳ级组(10例)。将样本进行软骨及软骨下骨分离后，运用实时定量PCR检测不同分组中软骨及软骨下骨中miR-214的表达情况。结果在所有软骨及软骨下骨中均能检测到miR-214的表达。比较4组之间miR-214的表达情况，发现4组软骨中miR-214的表达有明显差异(P＜0.05)，并且呈现逐渐升高的趋势；骨性关节炎发展的严重程度与miR-214的表达水平密切相关。Ⅲ级组中软骨下骨中的miR-214的表达量明显高于其他3组(P＜0.05)。结论 miR-214的表达水平与骨性关节炎发展的严重程度密切相关，表明miR-214可能与骨性关节炎的发生、发展有密切联系。
목적：탐토불동Kellgren-Lawrence X선분급적골성관절염환자슬관절연골급연골하골중MicroRNA-214(miR-214)적표체，위진일보연구miR-214재골성관절염발생화발전중적작용제공의거。방법수집절지환자급골성관절염전슬관절치환환자슬관절경골평태40례，근거K-L평분표준장기분위Ⅰ급조(10례)、Ⅱ급조(10례)、Ⅲ급조(10례)、Ⅳ급조(10례)。장양본진행연골급연골하골분리후，운용실시정량PCR검측불동분조중연골급연골하골중miR-214적표체정황。결과재소유연골급연골하골중균능검측도miR-214적표체。비교4조지간miR-214적표체정황，발현4조연골중miR-214적표체유명현차이(P＜0.05)，병차정현축점승고적추세；골성관절염발전적엄중정도여miR-214적표체수평밀절상관。Ⅲ급조중연골하골중적miR-214적표체량명현고우기타3조(P＜0.05)。결론 miR-214적표체수평여골성관절염발전적엄중정도밀절상관，표명miR-214가능여골성관절염적발생、발전유밀절련계。
Objective To detect the expression level of microRNA-214 (miR-214) in cartilage and subchondral bone of different graded osteoarthritis (OA), which was classified by X-ray diagnosis criteria of Kellgren and Lawrence, and provide theoretical foundation for researching the role of miR-214 in OA development.Methods Tibial plateaus of OA patients were collected from those who underwent total knee arthroplasty (TKA) and amputation. Forty samples were divided into 4 groups by K-L osteoarthritis scoring system: gradeⅠgroup (n=10), gradeⅡgroup (n=10), gradeⅢgroup (n=10), and gradeⅣgroup (n=10). After getting the samples, we segregated cartilage and subchondral bone, and the expression level of miR-214 by real-time PCR was tested.Results miR-214 was examined in all cartilage and subchondral bone. The expression level of miR-214 was compared in four groups, and it showed a rising trend with significant difference (P<0.05). Positive correlation was found between the severity of OA and the expression level of miR-214. Among the four groups, the level of miR-214 in subchondral bone was highest.Conclusion The expression level of miR-214 in 4 groups is keeping rising, which is related to the severity of OA progression. And the expression level of miR-214 also has obvious change in subchondral bone. Ourfindings indicate that miR-214 has intertwined relationship with the development and progression of OA, which provides theoretical foundation for OA development and treatment.