中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
41期
3357-3360
,共4页
周红%袁永胜%戚志强%佟晴%张克忠
週紅%袁永勝%慼誌彊%佟晴%張剋忠
주홍%원영성%척지강%동청%장극충
帕金森病%氧化应激%认知功能障碍
帕金森病%氧化應激%認知功能障礙
파금삼병%양화응격%인지공능장애
Parkinson's disease%Oxidative stress%Cognitive dysfunction
目的 探讨帕金森病(PD)患者血浆氧化应激指标的变化及其与认知功能的关系.方法 入选2012年5月至2013年6月南京医科大学第一附属医院门诊及住院PD患者72例,另入选年龄、性别相匹配的45名健康对照者.记录PD患者的性别、年龄、病程、受教育年限、Hoehn&Yahr (H-Y)分级,采用蒙特利尔认知评估量表(MoCA)北京版对PD患者及健康对照组的认知功能进行评估,使用酶联免疫吸附法(ELISA)检测血浆过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总谷胱甘肽(T-GSH)、丙二醛(MDA)水平,并对结果进行比较分析.结果 PD组血浆CAT、T-SOD、T-GSH水平明显低于健康对照组[(159±9) kU/L比(170±5) kU/L,P＜ 0.01;(97±24) kU/L比(124±25) kU/L,P＜ 0.01;(17±10)μmol/L比(60±51) μmol/L,P＜ 0.01];早期(H-Y分级I～Ⅱ级)PD组与中晚期(H-Y分级Ⅲ级及以上)PD组间血浆CAT、T-SOD、T-GSH、GSH-Px、MDA水平无差异(P＞0.05);但合并轻度认知功能障碍(MCI)的PD组血浆T-GSH水平低于无MCI的PD组[(14±7)μmoL/L比(19±11) μmol/L,P＜ 0.05];PD患者MoCA评分与受教育年限(β=0.634,P=0.000)、血浆T-GSH水平(β=0.204,P=0.014)呈正相关,与H-Y分级(β=-0.194,P=0.020)呈负相关.结论 血浆抗氧化机制的受损可能参与了PD患者的发病机制;血浆T-GSH水平的下降可能与PD患者的MCI具有相关性,其可能为PD患者合并认知功能障碍的潜在早期预测指标.
目的 探討帕金森病(PD)患者血漿氧化應激指標的變化及其與認知功能的關繫.方法 入選2012年5月至2013年6月南京醫科大學第一附屬醫院門診及住院PD患者72例,另入選年齡、性彆相匹配的45名健康對照者.記錄PD患者的性彆、年齡、病程、受教育年限、Hoehn&Yahr (H-Y)分級,採用矇特利爾認知評估量錶(MoCA)北京版對PD患者及健康對照組的認知功能進行評估,使用酶聯免疫吸附法(ELISA)檢測血漿過氧化氫酶(CAT)、總超氧化物歧化酶(T-SOD)、穀胱甘肽過氧化物酶(GSH-Px)、總穀胱甘肽(T-GSH)、丙二醛(MDA)水平,併對結果進行比較分析.結果 PD組血漿CAT、T-SOD、T-GSH水平明顯低于健康對照組[(159±9) kU/L比(170±5) kU/L,P＜ 0.01;(97±24) kU/L比(124±25) kU/L,P＜ 0.01;(17±10)μmol/L比(60±51) μmol/L,P＜ 0.01];早期(H-Y分級I～Ⅱ級)PD組與中晚期(H-Y分級Ⅲ級及以上)PD組間血漿CAT、T-SOD、T-GSH、GSH-Px、MDA水平無差異(P＞0.05);但閤併輕度認知功能障礙(MCI)的PD組血漿T-GSH水平低于無MCI的PD組[(14±7)μmoL/L比(19±11) μmol/L,P＜ 0.05];PD患者MoCA評分與受教育年限(β=0.634,P=0.000)、血漿T-GSH水平(β=0.204,P=0.014)呈正相關,與H-Y分級(β=-0.194,P=0.020)呈負相關.結論 血漿抗氧化機製的受損可能參與瞭PD患者的髮病機製;血漿T-GSH水平的下降可能與PD患者的MCI具有相關性,其可能為PD患者閤併認知功能障礙的潛在早期預測指標.
목적 탐토파금삼병(PD)환자혈장양화응격지표적변화급기여인지공능적관계.방법 입선2012년5월지2013년6월남경의과대학제일부속의원문진급주원PD환자72례,령입선년령、성별상필배적45명건강대조자.기록PD환자적성별、년령、병정、수교육년한、Hoehn&Yahr (H-Y)분급,채용몽특리이인지평고량표(MoCA)북경판대PD환자급건강대조조적인지공능진행평고,사용매련면역흡부법(ELISA)검측혈장과양화경매(CAT)、총초양화물기화매(T-SOD)、곡광감태과양화물매(GSH-Px)、총곡광감태(T-GSH)、병이철(MDA)수평,병대결과진행비교분석.결과 PD조혈장CAT、T-SOD、T-GSH수평명현저우건강대조조[(159±9) kU/L비(170±5) kU/L,P＜ 0.01;(97±24) kU/L비(124±25) kU/L,P＜ 0.01;(17±10)μmol/L비(60±51) μmol/L,P＜ 0.01];조기(H-Y분급I～Ⅱ급)PD조여중만기(H-Y분급Ⅲ급급이상)PD조간혈장CAT、T-SOD、T-GSH、GSH-Px、MDA수평무차이(P＞0.05);단합병경도인지공능장애(MCI)적PD조혈장T-GSH수평저우무MCI적PD조[(14±7)μmoL/L비(19±11) μmol/L,P＜ 0.05];PD환자MoCA평분여수교육년한(β=0.634,P=0.000)、혈장T-GSH수평(β=0.204,P=0.014)정정상관,여H-Y분급(β=-0.194,P=0.020)정부상관.결론 혈장항양화궤제적수손가능삼여료PD환자적발병궤제;혈장T-GSH수평적하강가능여PD환자적MCI구유상관성,기가능위PD환자합병인지공능장애적잠재조기예측지표.
Objective To study the changes of plasma levels of oxidative stress biomarkers in patients with parkinson's disease (PD),and to explore its association with cognition function.Methods Seventy-two PD patients from June 2013 to May 2012 were enrolled.All of them were outpatients or inpatients at the First Affiliated Hospital of Nanjing Medical University.And forty-five age-and gender-matched healthy subjects were used as controls.The information including gender,age,illness duration,years of education and Hoehn & Yahr (H-Y) stage were recorded.Cognition function of all the patients with PD and the controls were measured by using Montreal Cognitive Assessment (MoCA) scale.Plasma levels of catalase (CAT),total superoxide dismutase (T-SOD),glutathione peroxidase (GSH-Px),total glutathione (T-GSH) and malondialdehyde (MDA) were measured by ELISA.Then we compared and analyzed the results.Results Plasma levels of CAT,T-SOD and T-GSH in PD group were significantly lower than the control group[(159 ± 9) kU/L vs (170 ± 5) kU/L,P ＜ 0.01;(97 ± 24) kU/L vs (124 ± 25) kU/L,P ＜0.01;(17 ± 10) μmol/L vs (60 ±51) μmol/L,P ＜ 0.01].Plasma levels of CAT,T-GSH,GSH-Px and MDA were no differences between early PD group (H-Y stage Ⅰ-Ⅱ) and middle-late PD group (H-Y stage Ⅲ or higher) of patients (P ＞ 0.05).Plasma levels of T-GSH in PD group with mild cognitive impairment(MCI) were obviously lower than PD group without MCI [(14 ± 7) μmol/L vs (19 ± 11) μmol/L,P ＜ 0.05].In PD patients,MoCA scores were positively correlated with years of education (β =0.634,P =0.000) and plasma levels of T-GSH (β =0.204,P =0.014),and were negatively correlated with H-Y stage (β =-0.194,P =0.020).Conclusions The damage of plasma antioxidant mechanism may be involved in the pathogenesis of patients with PD.Decrease in plasma levels of T-GSH may be associated with MCI in PD patients.Plasma levels of T-GSH may be a potential early predictive index in PD patients with cognitive dysfunction.
