CAJ | 학술논문

目的探讨肝癌细胞(HepG2细胞系)在缺氧缺养内环境下对5种肝动脉化疗栓塞(TACE)术中常用化疗药物单药及双药联合的敏感性.方法 HepG2细胞分别在含化疗药的4种浓度缺氧缺养培养液中培养2、4、6、24h.MTT法测定各自抑瘤率.所用5种化疗药物分别为:吡柔比星、顺铂、丝裂霉素C、奥沙利铂、5-氟尿嘧啶.结果在4种缺氧缺养条件下均敏感的药物是:吡柔比星、丝裂霉素C、奥沙利铂.在氧浓度为5％、10％、25％的培养液中,5种化疗药作用24h的抑瘤率均明显高于2h的抑瘤率.在氧浓度50％培养液中,5种化疗药2时间亚组间的抑瘤率差异无统计学意义.与单药比较,双药联合组抑瘤率并无明显增加.结论吡柔比星、丝裂霉素C、奥沙利铂在不同缺氧缺养条件下均对HepG2细胞具有有效的毒性作用.在中重度缺氧缺养条件下,延长药物作用时间可以提高药物的抑瘤作用.而双药联合不能增强疗效.
목적 탐토간암세포(HepG2세포계)재결양결양내배경하대5충간동맥화료전새(TACE)술중상용화료약물단약급쌍약연합적민감성.방법 HepG2세포분별재함화료약적4충농도결양결양배양액중배양2、4、6、24h.MTT법측정각자억류솔.소용5충화료약물분별위:필유비성、순박、사렬매소C、오사리박、5-불뇨밀정.결과 재4충결양결양조건하균민감적약물시:필유비성、사렬매소C、오사리박.재양농도위5％、10％、25％적배양액중,5충화료약작용24h적억류솔균명현고우2h적억류솔.재양농도50％배양액중,5충화료약2시간아조간적억류솔차이무통계학의의.여단약비교,쌍약연합조억류솔병무명현증가.결론 필유비성、사렬매소C、오사리박재불동결양결양조건하균대HepG2세포구유유효적독성작용.재중중도결양결양조건하,연장약물작용시간가이제고약물적억류작용.이쌍약연합불능증강료효.
Objective To investigate whether 5 different chemotherapeutic drugs and their combination of either two drugs could further promote the inhibition on the cell growth of HCC cell line (HepG2) in vitro in the hypoxic and hyponutritional culture medium (HHCM) mimicking the different scenarios of transcatheter arterial chemoembolization (TACE).Methods The cells were treated by 5 drugs for 2 h, 4 h,6 h and 24 h, which include epirubicin (EPI), cisplatin (DDP), mitomycin-C (MMC), oxaliplatin (OXA) and 5-fluorouracil (5-FU) in four concentrations of HHCM (5％, 10％, 25％ and 50％) mimicking the scenarios during TACE and the cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.The combinations of dual drugs treated for 24 h were also tested.Results The sensitive drugs with inhibition rates more than 30％ were EPI, MMC and OXA in 4 different concentrations of HHCM.The sensitivity of the drugs treated for 24 h was significantly increased compared with that for 2 h in 5％, 10％ and 25％ HHCM.The dual combinations did not increase the chemosensitivity of HepG2 cells.Conclusions EPI, MMC and OXA exhibited cytotoxic activity against HepG2 cells in various hypoxia and hyponutrition states.Prolonging the exposure time could increase the sensitivity of drug in HHCM, and the combination of dual drugs cannot enhance the cytotoxic effect.