CAJ | 학술논문

天然产物抗肿瘤活性成分具有高效低毒和结构多样性。靶向筛选和分离能够提高活性化合物的分离效率，降低实验成本，缩短实验周期，并且减少大量使用有机试剂造成的环境污染。本文概述了近年来天然产物抗肿瘤活性成分的靶向筛选和分离的方法，包括以微管蛋白、端粒DNA的G－四联体、基质金属蛋白酶、DNA拓扑异构酶为靶点的活性成分筛选分离方法，以及利用脂筏色谱技术和计算机虚拟筛选技术等靶向筛选分离方法。
천연산물항종류활성성분구유고효저독화결구다양성。파향사선화분리능구제고활성화합물적분리효솔，강저실험성본，축단실험주기，병차감소대량사용유궤시제조성적배경오염。본문개술료근년래천연산물항종류활성성분적파향사선화분리적방법，포괄이미관단백、단립DNA적G－사련체、기질금속단백매、DNA탁복이구매위파점적활성성분사선분리방법，이급이용지벌색보기술화계산궤허의사선기술등파향사선분리방법。
Anti-tumor ingredients have the characteristics of efficient activity, low toxicity and structural diversity from natural prod-uct.Targeted screening and separating the antitumor activity of natural products can improve the separation efficiency, reduce costs, shorten the test cycle, and reduce the amount of organic reagents which has caused environmental pollution.This article reviewed meth-ods of targeted screening and separation anti-tumor activity of natural products in recent years, including using microtubules, G-quadruplex DNA, and matrix metalloproteinases, DNA topoisomerase as targets for screening and separating, and using lipid rafts chro-matographic separation technology and computer virtual screening techniques for targeting isolation.